TY - JOUR
T1 - Green tea polyphenols mitigate deterioration of bone microarchitecture in middle-aged female rats
AU - Shen, Chwan Li
AU - Yeh, James K.
AU - Stoecker, Barbara J.
AU - Chyu, Ming Chien
AU - Wang, Jia Sheng
N1 - Funding Information:
This study was supported by Lubbock Endowed Professorships, Lubbock, TX, USA (CLS) and the NIH/NCI grant CA90997 (JSW). We thank Julie Guerrieri for animal care and bone sample preparation of histomorphometry. We also thank Joe Hoefler for helping in the analysis of bone samples by µCT.
Funding Information:
Supported by Lubbock Endowed Professorship, Lubbock, Texas (CLS) and NIH/NCI grant CA90997 (JSW).
PY - 2009/4
Y1 - 2009/4
N2 - Our previous study demonstrated that green tea polyphenols (GTP) benefit bone health in middle-aged female rats without (sham, SH) and with ovariectomy (OVX), because of GTP's antioxidant capacity. The current study further evaluates whether GTP can restore bone micro-structure in both gonad-intact and gonadal-hormone-deficient middle-aged female rats. A 16-week study was performed based on a 2 (SH vs. OVX) × 3 (no GTP, 0.1% GTP, and 0.5% GTP in drinking water) factorial design using 14-month-old female rats (n = 10/group). An additional 10 rats were euthanized at the beginning of study to provide baseline parameters. Analysis using dual-energy X-ray absorptiometry, histomorphometry, and micro-computed tomography showed that GTP supplementation resulted in (a) increased trabecular volume, thickness, number, and bone formation of proximal tibia, periosteal bone formation rate of tibia shaft, and cortical thickness and area of femur, and (b) decreased trabecular separation and bone erosion of proximal tibia, and endocortical bone eroded surface of tibia shaft. We concluded that drinking water supplemented with GTP mitigated deterioration of bone microarchitecture in both intact and ovariectomized middle-aged female rats by suppressing bone erosion, enhancing bone formation, and modulating endocortical and cancellous bone compartments, resulting in a larger net bone volume.
AB - Our previous study demonstrated that green tea polyphenols (GTP) benefit bone health in middle-aged female rats without (sham, SH) and with ovariectomy (OVX), because of GTP's antioxidant capacity. The current study further evaluates whether GTP can restore bone micro-structure in both gonad-intact and gonadal-hormone-deficient middle-aged female rats. A 16-week study was performed based on a 2 (SH vs. OVX) × 3 (no GTP, 0.1% GTP, and 0.5% GTP in drinking water) factorial design using 14-month-old female rats (n = 10/group). An additional 10 rats were euthanized at the beginning of study to provide baseline parameters. Analysis using dual-energy X-ray absorptiometry, histomorphometry, and micro-computed tomography showed that GTP supplementation resulted in (a) increased trabecular volume, thickness, number, and bone formation of proximal tibia, periosteal bone formation rate of tibia shaft, and cortical thickness and area of femur, and (b) decreased trabecular separation and bone erosion of proximal tibia, and endocortical bone eroded surface of tibia shaft. We concluded that drinking water supplemented with GTP mitigated deterioration of bone microarchitecture in both intact and ovariectomized middle-aged female rats by suppressing bone erosion, enhancing bone formation, and modulating endocortical and cancellous bone compartments, resulting in a larger net bone volume.
KW - Dietary supplement
KW - Green tea
KW - Histomorphometry
KW - Micro-CT
KW - Osteoporosis
UR - http://www.scopus.com/inward/record.url?scp=61849093729&partnerID=8YFLogxK
U2 - 10.1016/j.bone.2008.11.018
DO - 10.1016/j.bone.2008.11.018
M3 - Article
C2 - 19118658
AN - SCOPUS:61849093729
SN - 8756-3282
VL - 44
SP - 684
EP - 690
JO - Bone
JF - Bone
IS - 4
ER -