Glycosylated major urinary protein of the house mouse: Characterization of its N-linked oligosaccharides

Yehia Mechref, Lukas Zidek, Weidong Ma, Milos V. Novotny

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A minor component of the major urinary protein complex of the house mouse was chromatographically isolated and ascertained to be a previously suspected glycoprotein. Using highly sensitive mass-spectrometric techniques for sequencing and linkage analysis, the N-linked oligosaccharides of this glycoprotein were characterized. They were determined to be of the complex type with a wide heterogeneity. The heterogeneity was due to both the degree of sialylation and the presence of galactose residues in either β(1-3) or β(1-4) linkages. The biantennary structures were the most pronounced glycans, while tri- and tetraantennary entities were minor.

Original languageEnglish
Pages (from-to)231-235
Number of pages5
JournalGlycobiology
Volume10
Issue number3
DOIs
StatePublished - Mar 2000

Keywords

  • House mouse
  • Mass spectrometry
  • Oligosaccharide sequencing
  • Pheromone-binding protein

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