Glycoprotein Enrichment Analytical Techniques: Advantages and Disadvantages

R. Zhu, L. Zacharias, K. M. Wooding, W. Peng, Y. Mechref

Research output: Chapter in Book/Report/Conference proceedingChapter

20 Scopus citations

Abstract

Protein glycosylation is one of the most important posttranslational modifications. Numerous biological functions are related to protein glycosylation. However, analytical challenges remain in the glycoprotein analysis. To overcome the challenges associated with glycoprotein analysis, many analytical techniques were developed in recent years. Enrichment methods were used to improve the sensitivity of detection, while HPLC and mass spectrometry methods were developed to facilitate the separation of glycopeptides/proteins and enhance detection, respectively. Fragmentation techniques applied in modern mass spectrometers allow the structural interpretation of glycopeptides/proteins, while automated software tools started replacing manual processing to improve the reliability and throughput of the analysis. In this chapter, the current methodologies of glycoprotein analysis were discussed. Multiple analytical techniques are compared, and advantages and disadvantages of each technique are highlighted.

Original languageEnglish
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages397-429
Number of pages33
DOIs
StatePublished - 2017

Publication series

NameMethods in Enzymology
Volume585
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Enrichment
  • Glycoprotein
  • Glycoproteomics
  • LC-MS/MS
  • Proteomics

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