Glycomic profiling of invasive and non-invasive breast cancer cells

John A. Goetz, Yehia Mechref, Pilsoo Kang, Meei Huey Jeng, Milos V. Novotny

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

Quantitative profiling of glycans with different structures appears essential for a better understanding of the cellular adhesion phenomena associated with malignant transformation and the underlying aberrant glycosylation of cancer cells. Using the recently developed glycomic techniques and mass-spectrometric measurements, we compare the N-linked and O-linked oligosaccharide profiles for different breast cancer cell lines with those of normal epithelial cells. Statistically significant differences in certain neutral, sialylated and fucosylated structures are readily discerned through quantitative measurements, indicating a potential of distinguishing invasive and non-invasive cancer attributes. The glycomic profile data cluster accordingly using Principal Component Analysis, verifying further glycobiological differences due to the differences between normal and cancer cell lines.

Original languageEnglish
Pages (from-to)117-131
Number of pages15
JournalGlycoconjugate Journal
Volume26
Issue number2
DOIs
StatePublished - Feb 2009

Keywords

  • Glycomics
  • Invasive and non-invasive breast cancer cells
  • MALDI MS
  • Permethylation

Fingerprint Dive into the research topics of 'Glycomic profiling of invasive and non-invasive breast cancer cells'. Together they form a unique fingerprint.

Cite this