TY - JOUR
T1 - Genotypic characterization of antimicrobial resistant salmonella spp. Strains from three poultry processing plants in colombia
AU - Ramirez-Hernandez, Alejandra
AU - Carrascal-Camacho, Ana K.
AU - Varón-García, Andrea
AU - Brashears, Mindy M.
AU - Sanchez-Plata, Marcos X.
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/3
Y1 - 2021/3
N2 - The poultry industry in Colombia has implemented several changes and measures in chicken processing to improve sanitary operations and control pathogens’ prevalence. However, there is no official in-plant microbial profile reference data currently available throughout the processing value chains. Hence, this research aimed to study the microbial profiles and the antimicro-bial resistance of Salmonella isolates in three plants. In total, 300 samples were collected in seven processing sites. Prevalence of Salmonella spp. and levels of Enterobacteriaceae were assessed. Ad-ditionally, whole-genome sequencing was conducted to characterize the isolated strains genotypi-cally. Overall, the prevalence of Salmonella spp. in each establishment was 77%, 58% and 80% for plant A, B, and C. The mean levels of Enterobacteriaceae in the chicken rinsates were 5.03, 5.74, and 6.41 log CFU/mL for plant A, B, and C. Significant reductions were identified in the counts of post-chilling rinsate samples; however, increased levels were found in chicken parts. There were six dis-tinct Salmonella spp. clusters with the predominant sequence types ST32 and ST28. The serotypes Infantis (54%) and Paratyphi B (25%) were the most commonly identified within the processing plants with a high abundance of antimicrobial resistance genes.
AB - The poultry industry in Colombia has implemented several changes and measures in chicken processing to improve sanitary operations and control pathogens’ prevalence. However, there is no official in-plant microbial profile reference data currently available throughout the processing value chains. Hence, this research aimed to study the microbial profiles and the antimicro-bial resistance of Salmonella isolates in three plants. In total, 300 samples were collected in seven processing sites. Prevalence of Salmonella spp. and levels of Enterobacteriaceae were assessed. Ad-ditionally, whole-genome sequencing was conducted to characterize the isolated strains genotypi-cally. Overall, the prevalence of Salmonella spp. in each establishment was 77%, 58% and 80% for plant A, B, and C. The mean levels of Enterobacteriaceae in the chicken rinsates were 5.03, 5.74, and 6.41 log CFU/mL for plant A, B, and C. Significant reductions were identified in the counts of post-chilling rinsate samples; however, increased levels were found in chicken parts. There were six dis-tinct Salmonella spp. clusters with the predominant sequence types ST32 and ST28. The serotypes Infantis (54%) and Paratyphi B (25%) were the most commonly identified within the processing plants with a high abundance of antimicrobial resistance genes.
KW - Antimicrobial resistance
KW - Chicken processing
KW - Colombia
KW - Microbial profile
KW - Salmonella
UR - http://www.scopus.com/inward/record.url?scp=85102416582&partnerID=8YFLogxK
U2 - 10.3390/foods10030491
DO - 10.3390/foods10030491
M3 - Article
AN - SCOPUS:85102416582
SN - 2304-8158
VL - 10
SP - 1
EP - 17
JO - Foods
JF - Foods
IS - 3
M1 - 491
ER -