TY - JOUR
T1 - Genotypic characterization of antimicrobial resistant salmonella spp. Strains from three poultry processing plants in colombia
AU - Ramirez-Hernandez, Alejandra
AU - Carrascal-Camacho, Ana K.
AU - Varón-García, Andrea
AU - Brashears, Mindy M.
AU - Sanchez-Plata, Marcos X.
N1 - Funding Information:
We would also like to express our gratitude to the International Cultural Center at Texas Tech University for the awarded International Research and Development Seed Grant that supports this research in Colombia. Institutional Review Board Statement: Not applicable.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/3
Y1 - 2021/3
N2 - The poultry industry in Colombia has implemented several changes and measures in chicken processing to improve sanitary operations and control pathogens’ prevalence. However, there is no official in-plant microbial profile reference data currently available throughout the processing value chains. Hence, this research aimed to study the microbial profiles and the antimicro-bial resistance of Salmonella isolates in three plants. In total, 300 samples were collected in seven processing sites. Prevalence of Salmonella spp. and levels of Enterobacteriaceae were assessed. Ad-ditionally, whole-genome sequencing was conducted to characterize the isolated strains genotypi-cally. Overall, the prevalence of Salmonella spp. in each establishment was 77%, 58% and 80% for plant A, B, and C. The mean levels of Enterobacteriaceae in the chicken rinsates were 5.03, 5.74, and 6.41 log CFU/mL for plant A, B, and C. Significant reductions were identified in the counts of post-chilling rinsate samples; however, increased levels were found in chicken parts. There were six dis-tinct Salmonella spp. clusters with the predominant sequence types ST32 and ST28. The serotypes Infantis (54%) and Paratyphi B (25%) were the most commonly identified within the processing plants with a high abundance of antimicrobial resistance genes.
AB - The poultry industry in Colombia has implemented several changes and measures in chicken processing to improve sanitary operations and control pathogens’ prevalence. However, there is no official in-plant microbial profile reference data currently available throughout the processing value chains. Hence, this research aimed to study the microbial profiles and the antimicro-bial resistance of Salmonella isolates in three plants. In total, 300 samples were collected in seven processing sites. Prevalence of Salmonella spp. and levels of Enterobacteriaceae were assessed. Ad-ditionally, whole-genome sequencing was conducted to characterize the isolated strains genotypi-cally. Overall, the prevalence of Salmonella spp. in each establishment was 77%, 58% and 80% for plant A, B, and C. The mean levels of Enterobacteriaceae in the chicken rinsates were 5.03, 5.74, and 6.41 log CFU/mL for plant A, B, and C. Significant reductions were identified in the counts of post-chilling rinsate samples; however, increased levels were found in chicken parts. There were six dis-tinct Salmonella spp. clusters with the predominant sequence types ST32 and ST28. The serotypes Infantis (54%) and Paratyphi B (25%) were the most commonly identified within the processing plants with a high abundance of antimicrobial resistance genes.
KW - Antimicrobial resistance
KW - Chicken processing
KW - Colombia
KW - Microbial profile
KW - Salmonella
UR - http://www.scopus.com/inward/record.url?scp=85102416582&partnerID=8YFLogxK
U2 - 10.3390/foods10030491
DO - 10.3390/foods10030491
M3 - Article
AN - SCOPUS:85102416582
VL - 10
SP - 1
EP - 17
JO - Foods
JF - Foods
SN - 2304-8158
IS - 3
M1 - 491
ER -