Genome engineering tools in plant synthetic biology

Krishan Mohan Rai, Kaushik Ghose, Anshulika Rai, Harpal Singh, Rakesh Srivastava, Venugopal Mendu

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Scopus citations

Abstract

Plant synthetic biology is a rapidly growing field of science which is expected to significantly contribute to the food, fiber, and fuel deficit because of fast increasing world population. By combining the fundamental understanding of plant biology with technological advancement and genetic engineering principles, plant synthetic biology is poised to play a major role in accelerating the development of novel agronomical traits. The recent development of efficient design and modeling-related computational tools, improved DNA synthesis and assembly technologies to generate complicated DNA constructs, and cutting-edge next-generation sequencing technologies have reduced many of the obstacles of plant synthetic biology. In this chapter we have discussed the principles, methods of synthetic biology, and tools that can be applied to engineer various stages of central dogma keeping plant synthetic biology in perspective. We have also focused on the future applications and challenges of plant synthetic biology.

Original languageEnglish
Title of host publicationCurrent Developments in Biotechnology and Bioengineering
Subtitle of host publicationSynthetic Biology, Cell Engineering and Bioprocessing Technologies
PublisherElsevier
Pages47-73
Number of pages27
ISBN (Electronic)9780444640857
ISBN (Print)9780444640864
DOIs
StatePublished - Jan 1 2018

Keywords

  • CRISPR
  • CRISPRi
  • DNA assembly
  • DNA synthesis
  • Epigenomic editing
  • Genetic engineering tools
  • Genome editing
  • Meganucleases
  • Plant synthetic biology
  • Promoter engineering
  • TALEN
  • Transcriptional regulation
  • Translational regulation
  • Zinc-finger nucleases

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