TY - JOUR
T1 - Genetic transformation of garlic (Allium sativum L.) by particle bombardment
AU - Robledo-Paz, Alejandrina
AU - Cabrera-Ponce, José Luis
AU - Villalobos-Arámbula, Víctor Manuel
AU - Herrera-Estrella, Luis
AU - Jofre-Garfias, Alba Estela
PY - 2004/10
Y1 - 2004/10
N2 - Microprojectile bombardment was used to introduce DNA into embryogenic callus of garlic (Allium sativum L.) and produce stably transformed garlic plants. Embryogenic calluses, derived from garlic cultivar 'GT96-1', were bombarded with plasmid DNA containing genes coding for hygromycin phosphotransferase and β-glucuronidase. Putatively transformed calluses were identified in the bombarded tissue after 4 months of selection on 20 mg·L-1 hygromycin B. The transgenic nature of the selected material was demonstrated by GUS histochemical assay and Southern blot hybridization analysis, and twenty transgenic plants were regenerated.
AB - Microprojectile bombardment was used to introduce DNA into embryogenic callus of garlic (Allium sativum L.) and produce stably transformed garlic plants. Embryogenic calluses, derived from garlic cultivar 'GT96-1', were bombarded with plasmid DNA containing genes coding for hygromycin phosphotransferase and β-glucuronidase. Putatively transformed calluses were identified in the bombarded tissue after 4 months of selection on 20 mg·L-1 hygromycin B. The transgenic nature of the selected material was demonstrated by GUS histochemical assay and Southern blot hybridization analysis, and twenty transgenic plants were regenerated.
KW - Biolistic
KW - Genetic engineering
KW - Somatic embryogenesis
KW - Tissue culture
KW - Transgenic plants
UR - http://www.scopus.com/inward/record.url?scp=5444228353&partnerID=8YFLogxK
U2 - 10.21273/hortsci.39.6.1208
DO - 10.21273/hortsci.39.6.1208
M3 - Article
AN - SCOPUS:5444228353
SN - 0018-5345
VL - 39
SP - 1208
EP - 1211
JO - HortScience
JF - HortScience
IS - 6
ER -