Genetic transformation of garlic (Allium sativum L.) by particle bombardment

Alejandrina Robledo-Paz, José Luis Cabrera-Ponce, Víctor Manuel Villalobos-Arámbula, Luis Herrera-Estrella, Alba Estela Jofre-Garfias

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Microprojectile bombardment was used to introduce DNA into embryogenic callus of garlic (Allium sativum L.) and produce stably transformed garlic plants. Embryogenic calluses, derived from garlic cultivar 'GT96-1', were bombarded with plasmid DNA containing genes coding for hygromycin phosphotransferase and β-glucuronidase. Putatively transformed calluses were identified in the bombarded tissue after 4 months of selection on 20 mg·L-1 hygromycin B. The transgenic nature of the selected material was demonstrated by GUS histochemical assay and Southern blot hybridization analysis, and twenty transgenic plants were regenerated.

Original languageEnglish
Pages (from-to)1208-1211
Number of pages4
Issue number6
StatePublished - Oct 2004


  • Biolistic
  • Genetic engineering
  • Somatic embryogenesis
  • Tissue culture
  • Transgenic plants


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