Gene expression profile of mechanically impacted bovine articular cartilage explants

P. S. Chan, A. E. Schlueter, P. M. Coussens, G. J.M. Rosa, R. C. Haut, M. W. Orth

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Traumatic injury to a joint can initiate cartilage degradation. Blunt trauma increases matrix damage and decreases proteoglycan synthesis in in vitro models. Few studies have investigated gene expression of articular cartilage (AC) following mechanical loading. Recent advances in microarray technology allow analysis of a number of genes, and may elucidate pathways of AC degradation. In the present study, we used a bovine cDNA microarray to determine how acute trauma of cartilage explants in the absence of underlying bone alters gene expression. Results indicate that at least 19 genes were differentially expressed at 3 h after trauma. Fourteen genes were up-regulated and five genes were down-regulated relative to control explants. The up-regulated genes included cytokine and chemokine receptors, enzymes, and molecules involved in signal transduction. Genes of adhesion molecules and apoptosis were down-regulated. The results of this study highlight the potential benefits of using a bovine cDNA microarray to study cartilage metabolism.

Original languageEnglish
Pages (from-to)1146-1151
Number of pages6
JournalJournal of Orthopaedic Research
Volume23
Issue number5
DOIs
StatePublished - Sep 2005

Keywords

  • Articular cartilage
  • Matrix metalloproteinase
  • Mechanical impact
  • Quantitative real-time polymerase chain reaction
  • cDNA microarray

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