GC-MS SPME profiling of rhizobacterial volatiles reveals prospective inducers of growth promotion and induced systemic resistance in plants

Mohamed A. Farag; Choong Min Ryu; Lloyd W. Sumner; Paul W. Paré

doi:10.1016/j.phytochem.2006.07.021

GC-MS SPME profiling of rhizobacterial volatiles reveals prospective inducers of growth promotion and induced systemic resistance in plants

Mohamed A. Farag, Choong Min Ryu, Lloyd W. Sumner, Paul W. Paré

Chemistry and Biochemistry

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286 Scopus citations

Abstract

Chemical and plant growth studies of Bacilli strains GB03 and IN937a revealed that the volatile components 2,3-butanediol and acetoin trigger plant growth promotion in Arabidopsis. Differences in growth promotion when cytokinin-signaling mutants are exposed to GB03 versus IN937a volatiles suggest a divergence in chemical signaling for these two bacterial strains. To provide a comprehensive chemical profile of bacterial volatiles emitted from these biologically active strains, headspace solid phase microextraction (SPME) coupled with software extraction of overlapping GC-separated components was employed. Ten volatile metabolites already reported from GB03 and IN937a were identified as well as 28 compounds not previously characterized. Most of the newly identified compounds were branched-chain alcohols released from IN937a, at much higher levels than in GB03. Principal component analysis clearly separated GB03 from IN937a, with GB03 producing higher amounts of 3-methyl-1-butanol, 2-methyl-1-butanol and butane-1-methoxy-3-methyl. The branched-chain alcohols share a similar functional motif to that of 2,3-butanediol and may afford alternative structural patterns for elicitors from bacterial sources.

Original language	English
Pages (from-to)	2262-2268
Number of pages	7
Journal	Phytochemistry
Volume	67
Issue number	20
DOIs	https://doi.org/10.1016/j.phytochem.2006.07.021
State	Published - Oct 2006

Keywords

3-Methyl-1-butanol
B. amyliquefaciens
Bacillus subtilis
Ehrlich pathway
Plant growth promoting rhizobacteria (PGPR)
Principal component analysis (PCA)
Solid-phase microextraction (SPME)
Volatile organic compound (VOC)

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10.1016/j.phytochem.2006.07.021

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@article{565242d6bb5a40fbaa3eaeefdc8212a5,

title = "GC-MS SPME profiling of rhizobacterial volatiles reveals prospective inducers of growth promotion and induced systemic resistance in plants",

abstract = "Chemical and plant growth studies of Bacilli strains GB03 and IN937a revealed that the volatile components 2,3-butanediol and acetoin trigger plant growth promotion in Arabidopsis. Differences in growth promotion when cytokinin-signaling mutants are exposed to GB03 versus IN937a volatiles suggest a divergence in chemical signaling for these two bacterial strains. To provide a comprehensive chemical profile of bacterial volatiles emitted from these biologically active strains, headspace solid phase microextraction (SPME) coupled with software extraction of overlapping GC-separated components was employed. Ten volatile metabolites already reported from GB03 and IN937a were identified as well as 28 compounds not previously characterized. Most of the newly identified compounds were branched-chain alcohols released from IN937a, at much higher levels than in GB03. Principal component analysis clearly separated GB03 from IN937a, with GB03 producing higher amounts of 3-methyl-1-butanol, 2-methyl-1-butanol and butane-1-methoxy-3-methyl. The branched-chain alcohols share a similar functional motif to that of 2,3-butanediol and may afford alternative structural patterns for elicitors from bacterial sources.",

keywords = "3-Methyl-1-butanol, B. amyliquefaciens, Bacillus subtilis, Ehrlich pathway, Plant growth promoting rhizobacteria (PGPR), Principal component analysis (PCA), Solid-phase microextraction (SPME), Volatile organic compound (VOC)",

author = "Farag, {Mohamed A.} and Ryu, {Choong Min} and Sumner, {Lloyd W.} and Par{\'e}, {Paul W.}",

note = "Funding Information: This work was supported in part by The Robert A. Welch Foundation (Grant D-1478), the Herman Frasch Foundation for Chemical Research, Samuel Roberts Nobel Foundation and the KRIBB Initiative Research Program.",

year = "2006",

month = oct,

doi = "10.1016/j.phytochem.2006.07.021",

language = "English",

volume = "67",

pages = "2262--2268",

journal = "Phytochemistry",

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number = "20",

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T1 - GC-MS SPME profiling of rhizobacterial volatiles reveals prospective inducers of growth promotion and induced systemic resistance in plants

AU - Farag, Mohamed A.

AU - Ryu, Choong Min

AU - Sumner, Lloyd W.

AU - Paré, Paul W.

N1 - Funding Information: This work was supported in part by The Robert A. Welch Foundation (Grant D-1478), the Herman Frasch Foundation for Chemical Research, Samuel Roberts Nobel Foundation and the KRIBB Initiative Research Program.

PY - 2006/10

Y1 - 2006/10

N2 - Chemical and plant growth studies of Bacilli strains GB03 and IN937a revealed that the volatile components 2,3-butanediol and acetoin trigger plant growth promotion in Arabidopsis. Differences in growth promotion when cytokinin-signaling mutants are exposed to GB03 versus IN937a volatiles suggest a divergence in chemical signaling for these two bacterial strains. To provide a comprehensive chemical profile of bacterial volatiles emitted from these biologically active strains, headspace solid phase microextraction (SPME) coupled with software extraction of overlapping GC-separated components was employed. Ten volatile metabolites already reported from GB03 and IN937a were identified as well as 28 compounds not previously characterized. Most of the newly identified compounds were branched-chain alcohols released from IN937a, at much higher levels than in GB03. Principal component analysis clearly separated GB03 from IN937a, with GB03 producing higher amounts of 3-methyl-1-butanol, 2-methyl-1-butanol and butane-1-methoxy-3-methyl. The branched-chain alcohols share a similar functional motif to that of 2,3-butanediol and may afford alternative structural patterns for elicitors from bacterial sources.

AB - Chemical and plant growth studies of Bacilli strains GB03 and IN937a revealed that the volatile components 2,3-butanediol and acetoin trigger plant growth promotion in Arabidopsis. Differences in growth promotion when cytokinin-signaling mutants are exposed to GB03 versus IN937a volatiles suggest a divergence in chemical signaling for these two bacterial strains. To provide a comprehensive chemical profile of bacterial volatiles emitted from these biologically active strains, headspace solid phase microextraction (SPME) coupled with software extraction of overlapping GC-separated components was employed. Ten volatile metabolites already reported from GB03 and IN937a were identified as well as 28 compounds not previously characterized. Most of the newly identified compounds were branched-chain alcohols released from IN937a, at much higher levels than in GB03. Principal component analysis clearly separated GB03 from IN937a, with GB03 producing higher amounts of 3-methyl-1-butanol, 2-methyl-1-butanol and butane-1-methoxy-3-methyl. The branched-chain alcohols share a similar functional motif to that of 2,3-butanediol and may afford alternative structural patterns for elicitors from bacterial sources.

KW - 3-Methyl-1-butanol

KW - B. amyliquefaciens

KW - Bacillus subtilis

KW - Ehrlich pathway

KW - Plant growth promoting rhizobacteria (PGPR)

KW - Principal component analysis (PCA)

KW - Solid-phase microextraction (SPME)

KW - Volatile organic compound (VOC)

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U2 - 10.1016/j.phytochem.2006.07.021

DO - 10.1016/j.phytochem.2006.07.021

M3 - Article

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AN - SCOPUS:33748785472

SN - 0031-9422

VL - 67

SP - 2262

EP - 2268

JO - Phytochemistry

JF - Phytochemistry

IS - 20

ER -

GC-MS SPME profiling of rhizobacterial volatiles reveals prospective inducers of growth promotion and induced systemic resistance in plants

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