Abstract
A novel method for Fmoc/tBu solution-phase peptide synthesis and the development of a new benzyl-type group-assisted purification (GAP) protecting group is reported. This GAP protecting group is utilized in place of a polymer support, facilitating C→N Fmoc peptide synthesis without chromatography or recrystallization. The GAP group can be added and removed in high yield, and was used to synthesize over 1 gram of the immunostimulant, thymopentin, in high overall yield (83 %) and purity (99 %). Fmoc/tBu solution-phase peptide synthesis and the development of a new benzyl-type group-assisted purification (GAP) protecting group is reported. This new GAP protecting group replaces a polymer support, facilitating C→N Fmoc peptide synthesis without chromatography or recrystallization. Over 1 gram of the immunostimulant, thymopentin, was synthesized in high overall yield (83 %) and purity (99 %).
Original language | English |
---|---|
Pages (from-to) | 1714-1719 |
Number of pages | 6 |
Journal | European Journal of Organic Chemistry |
Volume | 2016 |
Issue number | 9 |
DOIs | |
State | Published - Mar 1 2016 |
Keywords
- Amino acids
- GAP chemistry
- Peptides
- Protecting groups
- Synthetic methods