TY - JOUR
T1 - Fused‐silica capillaries with surface‐bound dextran layer crosslinked with diepoxypolyethylene glycol for capillary electrophoresis of biological substances at reduced electroosmotic flow
AU - Mechref, Yehia
AU - Rassi, Ziad El
PY - 1995
Y1 - 1995
N2 - This report is concerned with the introduction of novel surface modification involving the covalent attachment of branched, high molecular weight dextrans and subsequent crosslinking with polyether chains on the inner surface of fused silica capillaries with the aim of producing hydrophilic capillaries with reduced electroosmotic flow (EOF). Three different molecular mass dextrans, namely 45, 71 or 150 kDa were covalently attached to the capillary surface, and subsequently crosslinked with diepoxypolyethylene glycol (PEG). This chemistry produced stable coating over a wide range of conditions including high and low pH aqueous solutions. Moreover, the various dextran‐PEG‐coated capillaries exhibited reduced electroosmotic flow. These features of the dextran‐PEG coatings were useful for the separations of basic and acidic proteins, and provided high resolution separation for closely related acidic monosaccharides and sialooligosaccharides .
AB - This report is concerned with the introduction of novel surface modification involving the covalent attachment of branched, high molecular weight dextrans and subsequent crosslinking with polyether chains on the inner surface of fused silica capillaries with the aim of producing hydrophilic capillaries with reduced electroosmotic flow (EOF). Three different molecular mass dextrans, namely 45, 71 or 150 kDa were covalently attached to the capillary surface, and subsequently crosslinked with diepoxypolyethylene glycol (PEG). This chemistry produced stable coating over a wide range of conditions including high and low pH aqueous solutions. Moreover, the various dextran‐PEG‐coated capillaries exhibited reduced electroosmotic flow. These features of the dextran‐PEG coatings were useful for the separations of basic and acidic proteins, and provided high resolution separation for closely related acidic monosaccharides and sialooligosaccharides .
KW - Acidic monosaccharides
KW - Capillary coating
KW - Capillary electrophoresis
KW - Proteins
KW - Sialooligosaccharides
UR - http://www.scopus.com/inward/record.url?scp=0028924279&partnerID=8YFLogxK
U2 - 10.1002/elps.1150160199
DO - 10.1002/elps.1150160199
M3 - Article
C2 - 7588534
AN - SCOPUS:0028924279
SN - 0173-0835
VL - 16
SP - 617
EP - 624
JO - ELECTROPHORESIS
JF - ELECTROPHORESIS
IS - 1
ER -