Functional Interactions of Lanthanum and Phospholipase D with the Abscisic Acid Signaling Effectors VP1 and ABI1-1 in Rice Protoplasts

Srinivas S.L. Gampala, Dik Hagenbeek, Christopher D. Rock

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

cis,trans-Abscisic acid (ABA) plays an important role in plant growth and development, regulation of seed maturation, germination, and adaptation to environmental stresses. Knowledge of ABA mechanisms of action and the interactions of components required for ABA signal transduction is far from complete. Using transient gene expression in rice protoplasts, we observed additive and inhibitory effects between maize VP1 (Viviparous-1, a transcriptional activator) and a dominant-negative mutant protein phosphatase, ABI1-1 (ABA-insensitive-1-1), from Arabidopsis. Lanthanide ions were shown to be specific agonists of ABA-inducible gene expression and to interact synergistically with ABA and overexpressed VP1. Both VP1 and lanthanum activities could be antagonized by coexpression of ABI1-1, which demonstrates the specific ABA dependence of these effectors on ABA-regulated gene expression. We obtained pharmacological evidence that phospholipase D (PLD) functions in ABA-inducible gene expression in rice. Antagonism of ABA, VP1, and lanthanum synergy by 1-butanol, a specific inhibitor of PLD, was similar to the inhibition by coexpression of ABI1-1. These results demonstrate that ABA, VP1, lanthanum, PLD, and ABI1 are all involved in ABA-regulated gene expression and are consistent with an integrated model whereby La3+ acts upstream of PLD.

Original languageEnglish
Pages (from-to)9855-9860
Number of pages6
JournalJournal of Biological Chemistry
Volume276
Issue number13
DOIs
StatePublished - Mar 30 2001

Fingerprint Dive into the research topics of 'Functional Interactions of Lanthanum and Phospholipase D with the Abscisic Acid Signaling Effectors VP1 and ABI1-1 in Rice Protoplasts'. Together they form a unique fingerprint.

Cite this