Functional analysis of the promoter of the rice sucrose phosphate synthase gene (sps1)

Miguel Martínez-Trujillo, Tztzqui Chávez-Bárcenas, Verónica Limones-Briones, June Simpson, Luis Herrera-Estrella

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Sucrose phosphate synthase (SPS) is a key enzyme in the translocation of photoassimilates from sink-to-source tissues and the remobilization of reserves from storage tissues during seed germination. The tissue specific distribution of SPS is mainly determined by transcriptional mechanisms. To gain further insight into the mechanisms that regulate the transcriptional activity of SPS genes, we carried out a deletion analysis of the rice sps1 promoter using transient expression assays and transgenic rice plants. The sps1 promoter is atypical since it lacks recognizable TATA and initiator sequences near the transcription start site. It was found that the minimal active sps1 promoter consists of a 146bp 5′ flanking sequence that is capable of directing activity in photosynthetic tissues in rice. No positive or negative regulatory elements upstream to position -146 influencing expression in photosynthetic tissues were found. It was also observed that the sps1 promoter fragment containing 5′ sequences upstream to position -1830 conferred expression only in the scutellum of germinating seeds, whereas shorter promoter versions directed expression in the scutellum but also in the aleurone layer, suggesting the presence of a negative element upstream of position -1188 that suppresses expression in the aleurone. Gain of function experiments suggest that the negative element present upstream of position -1188 is necessary but not sufficient to suppress expression in the aleurone.

Original languageEnglish
Pages (from-to)131-140
Number of pages10
JournalPlant Science
Volume166
Issue number1
DOIs
StatePublished - Jan 2004

Keywords

  • Photoassimilates
  • Scutellum
  • Transgenic

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