TY - JOUR
T1 - Functional analysis of the promoter of the rice sucrose phosphate synthase gene (sps1)
AU - Martínez-Trujillo, Miguel
AU - Chávez-Bárcenas, Tztzqui
AU - Limones-Briones, Verónica
AU - Simpson, June
AU - Herrera-Estrella, Luis
N1 - Funding Information:
This research was supported in part by grants from the HHMI (75191-526901) and the Rockefeller Foundation (AS 9644) to L.H.-E. We are grateful to Beatriz Jimenez and Guillermo Corona for the sequencing work and oligonucleotide synthesis, Guadalupe Sanchez for dissecting the seeds, José Luis Cabrera for suggestions on transformation of rice and Juan Caballero for analysis of the rice genome.
PY - 2004/1
Y1 - 2004/1
N2 - Sucrose phosphate synthase (SPS) is a key enzyme in the translocation of photoassimilates from sink-to-source tissues and the remobilization of reserves from storage tissues during seed germination. The tissue specific distribution of SPS is mainly determined by transcriptional mechanisms. To gain further insight into the mechanisms that regulate the transcriptional activity of SPS genes, we carried out a deletion analysis of the rice sps1 promoter using transient expression assays and transgenic rice plants. The sps1 promoter is atypical since it lacks recognizable TATA and initiator sequences near the transcription start site. It was found that the minimal active sps1 promoter consists of a 146bp 5′ flanking sequence that is capable of directing activity in photosynthetic tissues in rice. No positive or negative regulatory elements upstream to position -146 influencing expression in photosynthetic tissues were found. It was also observed that the sps1 promoter fragment containing 5′ sequences upstream to position -1830 conferred expression only in the scutellum of germinating seeds, whereas shorter promoter versions directed expression in the scutellum but also in the aleurone layer, suggesting the presence of a negative element upstream of position -1188 that suppresses expression in the aleurone. Gain of function experiments suggest that the negative element present upstream of position -1188 is necessary but not sufficient to suppress expression in the aleurone.
AB - Sucrose phosphate synthase (SPS) is a key enzyme in the translocation of photoassimilates from sink-to-source tissues and the remobilization of reserves from storage tissues during seed germination. The tissue specific distribution of SPS is mainly determined by transcriptional mechanisms. To gain further insight into the mechanisms that regulate the transcriptional activity of SPS genes, we carried out a deletion analysis of the rice sps1 promoter using transient expression assays and transgenic rice plants. The sps1 promoter is atypical since it lacks recognizable TATA and initiator sequences near the transcription start site. It was found that the minimal active sps1 promoter consists of a 146bp 5′ flanking sequence that is capable of directing activity in photosynthetic tissues in rice. No positive or negative regulatory elements upstream to position -146 influencing expression in photosynthetic tissues were found. It was also observed that the sps1 promoter fragment containing 5′ sequences upstream to position -1830 conferred expression only in the scutellum of germinating seeds, whereas shorter promoter versions directed expression in the scutellum but also in the aleurone layer, suggesting the presence of a negative element upstream of position -1188 that suppresses expression in the aleurone. Gain of function experiments suggest that the negative element present upstream of position -1188 is necessary but not sufficient to suppress expression in the aleurone.
KW - Photoassimilates
KW - Scutellum
KW - Transgenic
UR - http://www.scopus.com/inward/record.url?scp=1642526727&partnerID=8YFLogxK
U2 - 10.1016/j.plantsci.2003.08.017
DO - 10.1016/j.plantsci.2003.08.017
M3 - Article
AN - SCOPUS:1642526727
SN - 0168-9452
VL - 166
SP - 131
EP - 140
JO - Plant Science
JF - Plant Science
IS - 1
ER -