TY - JOUR
T1 - FTIR microspectroscopy reveals fatty acid-induced biochemical changes in C. elegans
AU - Bouyanfif, Amal
AU - Liyanage, Sumedha
AU - Hequet, Eric
AU - Moustaid-Moussa, Naima
AU - Abidi, Noureddine
PY - 2019
Y1 - 2019
N2 - Fourier transform infrared microspectroscopy (FTIR) is a promising method for the analysis of biological samples. Recent studies reported that FTIR imaging allows determination of the distribution of several biomoleculesin a sample with no staining or extraction. In this study, FTIR was used to monitor biochemical changes in C.elegans nematodes cultured in nematode maintenance media (CeMM) without supplementation and with supplementationwith either a long chain polyunsatured omega 3 fatty acid, eicosapentaenoic acid (EPA) or a saturated fatty acid, palmitic acid (PA) at 100 μM. EPA is an omega 3 fatty acid with documented health benefits while PA is generally consumed in diets. Worms were placed on BaF2 slides, and FTIR spectra were collectedfrom single worms in transmission mode using a focal plane array detector. Principal component analysisgrouped the FTIR spectra into three clusters corresponding to spectra of worms cultured with no supplementation,worms cu
AB - Fourier transform infrared microspectroscopy (FTIR) is a promising method for the analysis of biological samples. Recent studies reported that FTIR imaging allows determination of the distribution of several biomoleculesin a sample with no staining or extraction. In this study, FTIR was used to monitor biochemical changes in C.elegans nematodes cultured in nematode maintenance media (CeMM) without supplementation and with supplementationwith either a long chain polyunsatured omega 3 fatty acid, eicosapentaenoic acid (EPA) or a saturated fatty acid, palmitic acid (PA) at 100 μM. EPA is an omega 3 fatty acid with documented health benefits while PA is generally consumed in diets. Worms were placed on BaF2 slides, and FTIR spectra were collectedfrom single worms in transmission mode using a focal plane array detector. Principal component analysisgrouped the FTIR spectra into three clusters corresponding to spectra of worms cultured with no supplementation,worms cu
M3 - Article
SP - 8
EP - 15
JO - Vibrational Spectroscopy
JF - Vibrational Spectroscopy
ER -