FTIR microspectroscopy reveals fatty acid-induced biochemical changes in C. elegans

Amal Bouyanfif, Sumedha Liyanage, Eric Hequet, Naima Moustaid-Moussa, Noureddine Abidi

Research output: Contribution to journalArticlepeer-review

Abstract

Fourier transform infrared microspectroscopy (FTIR) is a promising method for the analysis of biological samples. Recent studies reported that FTIR imaging allows determination of the distribution of several biomolecules<br>in a sample with no staining or extraction. In this study, FTIR was used to monitor biochemical changes in C.<br>elegans nematodes cultured in nematode maintenance media (CeMM) without supplementation and with supplementation<br>with either a long chain polyunsatured omega 3 fatty acid, eicosapentaenoic acid (EPA) or a saturated fatty acid, palmitic acid (PA) at 100 μM. EPA is an omega 3 fatty acid with documented health benefits while PA is generally consumed in diets. Worms were placed on BaF2 slides, and FTIR spectra were collected<br>from single worms in transmission mode using a focal plane array detector. Principal component analysis<br>grouped the FTIR spectra into three clusters corresponding to spectra of worms cultured with no supplementation,<br>worms cu
Original languageEnglish
Pages (from-to)8-15
JournalVibrational Spectroscopy
StatePublished - 2019

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