FTIR microspectroscopy reveals fatty acid-induced biochemical changes in C. elegans

Amal Bouyanfif; Sumedha Liyanage; Eric Hequet; Naima Moustaid-Moussa; Noureddine Abidi

FTIR microspectroscopy reveals fatty acid-induced biochemical changes in C. elegans

Amal Bouyanfif, Sumedha Liyanage, Eric Hequet, Naima Moustaid-Moussa, Noureddine Abidi

Research output: Contribution to journal › Article › peer-review

Abstract

Fourier transform infrared microspectroscopy (FTIR) is a promising method for the analysis of biological samples. Recent studies reported that FTIR imaging allows determination of the distribution of several biomolecules in a sample with no staining or extraction. In this study, FTIR was used to monitor biochemical changes in C. elegans nematodes cultured in nematode maintenance media (CeMM) without supplementation and with supplementation with either a long chain polyunsatured omega 3 fatty acid, eicosapentaenoic acid (EPA) or a saturated fatty acid, palmitic acid (PA) at 100 μM. EPA is an omega 3 fatty acid with documented health benefits while PA is generally consumed in diets. Worms were placed on BaF2 slides, and FTIR spectra were collected from single worms in transmission mode using a focal plane array detector. Principal component analysis grouped the FTIR spectra into three clusters corresponding to spectra of worms cultured with no supplementation, worms cu

Original language	English
Pages (from-to)	8-15
Journal	Vibrational Spectroscopy
State	Published - 2019

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abstract = "Fourier transform infrared microspectroscopy (FTIR) is a promising method for the analysis of biological samples. Recent studies reported that FTIR imaging allows determination of the distribution of several biomoleculesin a sample with no staining or extraction. In this study, FTIR was used to monitor biochemical changes in C.elegans nematodes cultured in nematode maintenance media (CeMM) without supplementation and with supplementationwith either a long chain polyunsatured omega 3 fatty acid, eicosapentaenoic acid (EPA) or a saturated fatty acid, palmitic acid (PA) at 100 μM. EPA is an omega 3 fatty acid with documented health benefits while PA is generally consumed in diets. Worms were placed on BaF2 slides, and FTIR spectra were collectedfrom single worms in transmission mode using a focal plane array detector. Principal component analysisgrouped the FTIR spectra into three clusters corresponding to spectra of worms cultured with no supplementation,worms cu",

author = "Amal Bouyanfif and Sumedha Liyanage and Eric Hequet and Naima Moustaid-Moussa and Noureddine Abidi",

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T1 - FTIR microspectroscopy reveals fatty acid-induced biochemical changes in C. elegans

AU - Bouyanfif, Amal

AU - Liyanage, Sumedha

AU - Hequet, Eric

AU - Moustaid-Moussa, Naima

AU - Abidi, Noureddine

PY - 2019

Y1 - 2019

N2 - Fourier transform infrared microspectroscopy (FTIR) is a promising method for the analysis of biological samples. Recent studies reported that FTIR imaging allows determination of the distribution of several biomoleculesin a sample with no staining or extraction. In this study, FTIR was used to monitor biochemical changes in C.elegans nematodes cultured in nematode maintenance media (CeMM) without supplementation and with supplementationwith either a long chain polyunsatured omega 3 fatty acid, eicosapentaenoic acid (EPA) or a saturated fatty acid, palmitic acid (PA) at 100 μM. EPA is an omega 3 fatty acid with documented health benefits while PA is generally consumed in diets. Worms were placed on BaF2 slides, and FTIR spectra were collectedfrom single worms in transmission mode using a focal plane array detector. Principal component analysisgrouped the FTIR spectra into three clusters corresponding to spectra of worms cultured with no supplementation,worms cu

AB - Fourier transform infrared microspectroscopy (FTIR) is a promising method for the analysis of biological samples. Recent studies reported that FTIR imaging allows determination of the distribution of several biomoleculesin a sample with no staining or extraction. In this study, FTIR was used to monitor biochemical changes in C.elegans nematodes cultured in nematode maintenance media (CeMM) without supplementation and with supplementationwith either a long chain polyunsatured omega 3 fatty acid, eicosapentaenoic acid (EPA) or a saturated fatty acid, palmitic acid (PA) at 100 μM. EPA is an omega 3 fatty acid with documented health benefits while PA is generally consumed in diets. Worms were placed on BaF2 slides, and FTIR spectra were collectedfrom single worms in transmission mode using a focal plane array detector. Principal component analysisgrouped the FTIR spectra into three clusters corresponding to spectra of worms cultured with no supplementation,worms cu

M3 - Article

SP - 8

EP - 15

JO - Vibrational Spectroscopy

JF - Vibrational Spectroscopy

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