FTIR imaging detects diet and genotype-dependent chemical composition changes in wild type and mutant: C. elegans strains: C. elegans strains

© This journal is The Royal Society of Chemistry. This study focuses on the use of Fourier Transform Infrared (FTIR) microspectroscopy to determine chemical changes induced in the nematode Caenorhabditis elegans by supplementation of C. elegans maintenance media (CeMM) by Eicosapentaenoic acid (EPA). Wild-Type C. elegans (N2) and mutant strains (tub-1 and fat-3) were grown in CeMM alone, and CeMM supplemented with EPA at 25 or 100 μM. Feeding was performed for 72 h. FTIR imaging was performed in transmission mode on individual worms. The FTIR imaging analysis of wild-Type animals revealed the presence of vibrations assigned to unsaturated fatty acids, specifically bands at 3008 cm -1 (C-H, olefinic stretch) and 1744 cm -1 (CO, unsaturated fatty acids). It confirmed previously reported synthesis of unsaturated fatty acids in wild-Type C. elegans. For the FTIR spectra of mutant strains, these vibrations were absent or present only as very small shoulder, which indicates that tub-1 and f