Fluorescent Microspheres Test for Evaluation of Oviductal Patency

Sofie Sitters, John J. Dascanio

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Blockage of one or both oviducts can result in reduction of fertility in the mare. Fluorescent microspheres have been used as an alternative to evaluate oviductal patency. A solution containing fluorescent microspheres is deposited onto the surface of the ovary using a transvaginal ultrasound probe and needle or applied into the proximal oviductal lumen via a laparoscope. This technique requires access to either a fluorescent microscope or a flow cytometer. This chapter discusses the step by step procedure for performing transvaginal ultrasound technique and laparoscope technique, and outlines the equipment and supplies needed for these techniques. Transvaginal deposition may result in a variable amount of beads entering the infundibulum; whereas, laparoscopic cannulation results in more accurate placement of beads. Laparoscopic deposition of beads also allows for direct observation of the oviduct.

Original languageEnglish
Title of host publicationEquine Reproductive Procedures
Number of pages2
ISBN (Electronic)9781118904398
ISBN (Print)9780470960394
StatePublished - Aug 25 2014


  • Fluorescent microspheres
  • Laparoscope technique
  • Oviductal patency
  • Pregnant mare
  • Transvaginal ultrasound technique


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