Fluorescence correlation spectroscopy: A review of biochemical and microfluidic applications

Yu Tian, Michelle M. Martinez, Dimitri Pappas

Research output: Contribution to journalReview articlepeer-review

64 Scopus citations


Over the years fluorescence correlation spectroscopy (FCS) has proven to be a useful technique that has been utilized in several fields of study. Although FCS initially suffered from poor signal-to-noise ratios, the incorporation of confocal microscopy has overcome this drawback and transformed FCS into a sensitive technique with high figures of merit. In addition, tandem methods have evolved to include dual-color cross-correlation, total internal reflection fluorescence correlation, and fluorescence lifetime correlation spectroscopy combined with time-correlated single-photon counting. In this review, we discuss several applications of FSC for biochemical, microfluidic, and cellular investigations.

Original languageEnglish
Pages (from-to)115A-124A
JournalApplied Spectroscopy
Issue number4
StatePublished - Apr 2011


  • Bioanalysis
  • Confocal microscopy
  • FCS
  • Fluorescence correlation spectroscopy
  • Hydrodynamics
  • Microfluids


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