@article{2128e3b352ce42cba36052046ac837dd,
title = "Ferredoxin/ferredoxin-thioredoxin reductase complex: Complete NMR mapping of the interaction site on ferredoxin by gallium substitution",
abstract = "The reduction of ferredoxin-thioredoxin reductase (FTR) by plant-type ferredoxin plays an important role in redox regulation in plants and cyanobacteria. Nuclear magnetic resonance (NMR) was used to map the binding sites on Synechocystis ferredoxin for FTR. A gallium-substituted structural analog of this [2Fe-2S] ferredoxin was obtained by reconstituting the apoprotein in a refolding buffer containing gallium. For the first time, the complete interaction interface of a [2Fe-2S] ferredoxin with a target enzyme has been mapped by NMR chemical shift perturbation with this diamagnetic structural analog.",
keywords = "Chemical shift perturbation, Ferredoxin-thioredoxin reductase, [2Fe-2S] Ferredoxin",
author = "Xingfu Xu and Kim, {Sung Kun} and Peter Sch{\"u}rmann and Masakazu Hirasawa and Tripathy, {Jatindra N.} and Jody Smith and Knaff, {David B.} and Marcellus Ubbink",
note = "Funding Information: This project was supported by grants from the US Department of Energy (DE-FG03-99ER20346 to DBK), the Schweizerischer National fonds (to P.S.) and the Netherlands Organization for Scientific Research (700.52.425 to M.U.). J.S. was supported by an undergraduate research fellowship from the Howard Hughes Medical Institute. The authors thank Dr. Shaodong Dai for the coordinates of the FTR/Fd crystal structure, Mr. J. van Brussel for Ga element analysis, Prof. Michael K. Johnson (Department of Chemistry, University of Georgia) for his suggestions about the use of Ga-substituted ferredoxin and Dr. Bernard Lagoutte (Department of Biophysics, C.E.A. Saclay) for his kind gift of the plasmid used to express ferredoxin. ",
year = "2006",
month = dec,
day = "11",
doi = "10.1016/j.febslet.2006.11.027",
language = "English",
volume = "580",
pages = "6714--6720",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "FEBS letters",
number = "28-29",
}