TY - JOUR
T1 - Fate of Selectable Marker DNA Integrated into the Genome of Nicotiana tabacum
AU - Czernilofsky, Armin P.
AU - Hain, Rüdiger
AU - Herrera-Estrella, Luis
AU - Lörz, Horst
AU - Goyvaerts, Elisabeth
AU - Baker, Barbara J.
AU - Schell, Jeff
PY - 1986/4
Y1 - 1986/4
N2 - To compare the effects of different transformation methods on the integration behavior and structural stability of integrated foreign genes in plant cells, tobacco protoplasts were transformed with Escherichia coli plasmid pLGV2103neo DNA using the Ca phosphate DNA coprecipitation technique. Parallel transformations were done by cocultivation with Agrobacterium tumefaciens harboring the Ti plasmid derivatives pGV3850::2103neo or pGV3850::1103neo. A comparison of the fine structure of the integrated donor DNA obtained by direct gene transfer and by cocultivation indicates that the donor DNA in cells transformed by the former technique undergoes structural changes and concatemerizations, while the DNA integrated by the latter procedure is often unaltered. The cotransformed nopaline synthase gene, which is present in the donor Ti plasmid DNA, was inactivated in two out of nine cases. Once integrated, the arrays of selectable marker DNA appear to be structurally stable under different cell culture and selection conditions, as well as after genetic transmission.
AB - To compare the effects of different transformation methods on the integration behavior and structural stability of integrated foreign genes in plant cells, tobacco protoplasts were transformed with Escherichia coli plasmid pLGV2103neo DNA using the Ca phosphate DNA coprecipitation technique. Parallel transformations were done by cocultivation with Agrobacterium tumefaciens harboring the Ti plasmid derivatives pGV3850::2103neo or pGV3850::1103neo. A comparison of the fine structure of the integrated donor DNA obtained by direct gene transfer and by cocultivation indicates that the donor DNA in cells transformed by the former technique undergoes structural changes and concatemerizations, while the DNA integrated by the latter procedure is often unaltered. The cotransformed nopaline synthase gene, which is present in the donor Ti plasmid DNA, was inactivated in two out of nine cases. Once integrated, the arrays of selectable marker DNA appear to be structurally stable under different cell culture and selection conditions, as well as after genetic transmission.
UR - http://www.scopus.com/inward/record.url?scp=0022705395&partnerID=8YFLogxK
U2 - 10.1089/dna.1986.5.101
DO - 10.1089/dna.1986.5.101
M3 - Article
C2 - 3519133
AN - SCOPUS:0022705395
SN - 0198-0238
VL - 5
SP - 101
EP - 113
JO - DNA
JF - DNA
IS - 2
ER -