Fast liquid chromatography separation and multiple-reaction monitoring mass spectrometric detection of neurotransmitters

Loubna A. Hammad, Matthew Neely, Bob Bridge, Yehia Mechref

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

We describe here the fast LC-MS/MS separation of a mixture of neurotransmitters consisting of dopamine, epinephrine, norepinephrine, 3,4-dihydroxybenzylamine (DHBA), salsolinol, serotonin, and γ-aminobutyric acid (GABA). The new UltiMate® 3000 Rapid Separation system (RSLC) was successfully coupled to the 4000 QTRAP mass spectrometer operating in multiple-reaction monitoring (MRM) mode. The separation was attained using a 100 mm length, 2.2 lm particle size Acclaim column at a flow rate of 0.5 mL/min. The column back pressure was 350 bar, while the total run time including column re-equilibration was 5.2 min. The peak resolution was minimally affected by the fast separation. The RSLC-MRM separation was found to have a precision range based on peak area for 50 replicate runs of 2-5% CV for all analytes, and the reproducibility of the retention time for all analytes was found to range from 0-2% CV. The described method represents an almost seven times shorter analysis time of neurotransmitters using LC/MRM which is very useful in screening large quantities of biological samples for various neurotransmitters.

Original languageEnglish
Pages (from-to)2369-2376
Number of pages8
JournalJournal of Separation Science
Volume32
Issue number14
DOIs
StatePublished - 2009

Keywords

  • Fast LC
  • Multi-reaction monitoring MS
  • Neurotransmitters

Fingerprint Dive into the research topics of 'Fast liquid chromatography separation and multiple-reaction monitoring mass spectrometric detection of neurotransmitters'. Together they form a unique fingerprint.

Cite this