TY - JOUR
T1 - Fast liquid chromatography separation and multiple-reaction monitoring mass spectrometric detection of neurotransmitters
AU - Hammad, Loubna A.
AU - Neely, Matthew
AU - Bridge, Bob
AU - Mechref, Yehia
N1 - Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2009
Y1 - 2009
N2 - We describe here the fast LC-MS/MS separation of a mixture of neurotransmitters consisting of dopamine, epinephrine, norepinephrine, 3,4-dihydroxybenzylamine (DHBA), salsolinol, serotonin, and γ-aminobutyric acid (GABA). The new UltiMate® 3000 Rapid Separation system (RSLC) was successfully coupled to the 4000 QTRAP mass spectrometer operating in multiple-reaction monitoring (MRM) mode. The separation was attained using a 100 mm length, 2.2 lm particle size Acclaim column at a flow rate of 0.5 mL/min. The column back pressure was 350 bar, while the total run time including column re-equilibration was 5.2 min. The peak resolution was minimally affected by the fast separation. The RSLC-MRM separation was found to have a precision range based on peak area for 50 replicate runs of 2-5% CV for all analytes, and the reproducibility of the retention time for all analytes was found to range from 0-2% CV. The described method represents an almost seven times shorter analysis time of neurotransmitters using LC/MRM which is very useful in screening large quantities of biological samples for various neurotransmitters.
AB - We describe here the fast LC-MS/MS separation of a mixture of neurotransmitters consisting of dopamine, epinephrine, norepinephrine, 3,4-dihydroxybenzylamine (DHBA), salsolinol, serotonin, and γ-aminobutyric acid (GABA). The new UltiMate® 3000 Rapid Separation system (RSLC) was successfully coupled to the 4000 QTRAP mass spectrometer operating in multiple-reaction monitoring (MRM) mode. The separation was attained using a 100 mm length, 2.2 lm particle size Acclaim column at a flow rate of 0.5 mL/min. The column back pressure was 350 bar, while the total run time including column re-equilibration was 5.2 min. The peak resolution was minimally affected by the fast separation. The RSLC-MRM separation was found to have a precision range based on peak area for 50 replicate runs of 2-5% CV for all analytes, and the reproducibility of the retention time for all analytes was found to range from 0-2% CV. The described method represents an almost seven times shorter analysis time of neurotransmitters using LC/MRM which is very useful in screening large quantities of biological samples for various neurotransmitters.
KW - Fast LC
KW - Multi-reaction monitoring MS
KW - Neurotransmitters
UR - http://www.scopus.com/inward/record.url?scp=70349183962&partnerID=8YFLogxK
U2 - 10.1002/jssc.200900158
DO - 10.1002/jssc.200900158
M3 - Article
C2 - 19569096
AN - SCOPUS:70349183962
VL - 32
SP - 2369
EP - 2376
JO - Journal of Separation Science
JF - Journal of Separation Science
SN - 1615-9306
IS - 14
ER -