Facile functionalization of Ag@SiO2 core-shell metal enhanced fluorescence nanoparticles for cell labeling

Meicong Dong, Yu Tian, Dimitri Pappas

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


We describe a versatile approach for functionalizing core-shell Ag@SiO 2 nanoparticles for live-cell imaging. The approach uses physical adsorption and does not need covalent linkage to synthesize antibody-based labels. The surface orientation is not controlled in this approach, but the signal enhancement is strong and consistent. Antibodies were then attached using a non-covalent process that takes advantage of biotin-avidin affinity. Metal-enhanced nanoparticles doped with rhodamine B were used as the luminescent reporter. The enhancement of rhodamine B was between 2.7 and 6.8 times. We demonstrated labeling of CD19+ Ramos B lymphocytes and CD4+ HuT 78 T lymphocytes using anti-CD19 and anti-CD4 nanocomposite labels, respectively. This physical adsorption process can accommodate a variety of fluorophore types, and has broad potential in bioanalytical and biosensing applications.

Original languageEnglish
Pages (from-to)1598-1602
Number of pages5
JournalAnalytical Methods
Issue number5
StatePublished - Mar 7 2014


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