TY - GEN
T1 - Fabrication of a graded micropillar surface for guided cell migration
AU - Krishnamoorthy, Srikumar
AU - Xu, Changxue
N1 - Publisher Copyright:
Copyright © 2020 ASME.
PY - 2020
Y1 - 2020
N2 - The migration of cells is caused by the interaction of cells and the local microenvironment around them, such as changes in stiffness, chemical gradients etc. The local topography of substrates in contact with cells is a key factor that regulates the migration of cells. The interaction between the topography of the substrate and cells is crucial for the understanding of tissue development and regeneration. In this paper, the fabrication of a graded micropillar substrate for studying topography-based cell migration is described in detail. The fabrication protocol comprises of the utilization of dynamic maskless lithography system, capillary molding, and corona arc surface treatment. The fabricated micropillar substrate has been shown and the cells have been successfully seeded on the substrate. Guided cell migration on the substrate with graded microtopography has been demonstrated to occur from the sparser zone to the denser zone. Moreover, some examples of potential applications are provided.
AB - The migration of cells is caused by the interaction of cells and the local microenvironment around them, such as changes in stiffness, chemical gradients etc. The local topography of substrates in contact with cells is a key factor that regulates the migration of cells. The interaction between the topography of the substrate and cells is crucial for the understanding of tissue development and regeneration. In this paper, the fabrication of a graded micropillar substrate for studying topography-based cell migration is described in detail. The fabrication protocol comprises of the utilization of dynamic maskless lithography system, capillary molding, and corona arc surface treatment. The fabricated micropillar substrate has been shown and the cells have been successfully seeded on the substrate. Guided cell migration on the substrate with graded microtopography has been demonstrated to occur from the sparser zone to the denser zone. Moreover, some examples of potential applications are provided.
KW - Graded micropillar
KW - Guided cell interaction
KW - Microtopography
UR - http://www.scopus.com/inward/record.url?scp=85100927646&partnerID=8YFLogxK
U2 - 10.1115/MSEC2020-8332
DO - 10.1115/MSEC2020-8332
M3 - Conference contribution
AN - SCOPUS:85100927646
T3 - ASME 2020 15th International Manufacturing Science and Engineering Conference, MSEC 2020
BT - Additive Manufacturing; Advanced Materials Manufacturing; Biomanufacturing; Life Cycle Engineering; Manufacturing Equipment and Automation
PB - American Society of Mechanical Engineers
T2 - ASME 2020 15th International Manufacturing Science and Engineering Conference, MSEC 2020
Y2 - 3 September 2020
ER -