TY - JOUR
T1 - Extensive intraspecific gene order and gene structural variations between Mo17 and other maize genomes
AU - Sun, Silong
AU - Zhou, Yingsi
AU - Chen, Jian
AU - Shi, Junpeng
AU - Zhao, Haiming
AU - Zhao, Hainan
AU - Song, Weibin
AU - Zhang, Mei
AU - Cui, Yang
AU - Dong, Xiaomei
AU - Liu, Han
AU - Ma, Xuxu
AU - Jiao, Yinping
AU - Wang, Bo
AU - Wei, Xuehong
AU - Stein, Joshua C.
AU - Glaubitz, Jeff C.
AU - Lu, Fei
AU - Yu, Guoliang
AU - Liang, Chengzhi
AU - Fengler, Kevin
AU - Li, Bailin
AU - Rafalski, Antoni
AU - Schnable, Patrick S.
AU - Ware, Doreen H.
AU - Buckler, Edward S.
AU - Lai, Jinsheng
N1 - Publisher Copyright:
© 2018, The Author(s).
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2018/9/1
Y1 - 2018/9/1
N2 - Maize is an important crop with a high level of genome diversity and heterosis. The genome sequence of a typical female line, B73, was previously released. Here, we report a de novo genome assembly of a corresponding male representative line, Mo17. More than 96.4% of the 2,183 Mb assembled genome can be accounted for by 362 scaffolds in ten pseudochromosomes with 38,620 annotated protein-coding genes. Comparative analysis revealed large gene-order and gene structural variations: approximately 10% of the annotated genes were mutually nonsyntenic, and more than 20% of the predicted genes had either large-effect mutations or large structural variations, which might cause considerable protein divergence between the two inbred lines. Our study provides a high-quality reference-genome sequence of an important maize germplasm, and the intraspecific gene order and gene structural variations identified should have implications for heterosis and genome evolution.
AB - Maize is an important crop with a high level of genome diversity and heterosis. The genome sequence of a typical female line, B73, was previously released. Here, we report a de novo genome assembly of a corresponding male representative line, Mo17. More than 96.4% of the 2,183 Mb assembled genome can be accounted for by 362 scaffolds in ten pseudochromosomes with 38,620 annotated protein-coding genes. Comparative analysis revealed large gene-order and gene structural variations: approximately 10% of the annotated genes were mutually nonsyntenic, and more than 20% of the predicted genes had either large-effect mutations or large structural variations, which might cause considerable protein divergence between the two inbred lines. Our study provides a high-quality reference-genome sequence of an important maize germplasm, and the intraspecific gene order and gene structural variations identified should have implications for heterosis and genome evolution.
UR - http://www.scopus.com/inward/record.url?scp=85051142685&partnerID=8YFLogxK
U2 - 10.1038/s41588-018-0182-0
DO - 10.1038/s41588-018-0182-0
M3 - Article
C2 - 30061735
AN - SCOPUS:85051142685
VL - 50
SP - 1289
EP - 1295
JO - Nature Genetics
JF - Nature Genetics
SN - 1061-4036
IS - 9
ER -