Expression of the Arabidopsis vacuolar H+-pyrophosphatase gene AVP1 in peanut to improve drought and salt tolerance

Hua Qin, Qiang Gu, Sundaram Kuppu, Li Sun, Xunlu Zhu, Neelam Mishra, Rongbin Hu, Guoxin Shen, Junling Zhang, Yizheng Zhang, Longfu Zhu, Xianlong Zhang, Mark Burow, Paxton Payton, Hong Zhang

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

The Arabidopsis gene AVP1 encodes an H+-pyrophosphatase that functions as a proton pump at the vacuolar membranes, generating a proton gradient across vacuolar membranes, which serves as the driving force for many secondary transporters on vacuolar membranes such as Na+/H+-antiporters. Overexpression of AVP1 could improve drought tolerance and salt tolerance in transgenic plants, suggesting a possible way in improving drought and salt tolerance in crops. The AVP1 was therefore introduced into peanut by Agrobacterium-mediated transformation. Analysis of AVP1-expressing peanut indicated that AVP1-overexpression in peanut could improve both drought and salt tolerance in greenhouse and growth chamber conditions, as AVP1-overexpressing peanuts produced more biomass and maintained higher photosynthetic rates under both drought and salt conditions. In the field, AVP1-overexpressing peanuts also outperformed wild-type plants by having higher photosynthetic rates and producing higher yields under low irrigation conditions.

Original languageEnglish
Pages (from-to)345-355
Number of pages11
JournalPlant Biotechnology Reports
Volume7
Issue number3
DOIs
StatePublished - Jul 2013

Keywords

  • Drought tolerance
  • Peanut transformation
  • Salt resistance
  • Transgenic plants
  • Yield improvement

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    Qin, H., Gu, Q., Kuppu, S., Sun, L., Zhu, X., Mishra, N., Hu, R., Shen, G., Zhang, J., Zhang, Y., Zhu, L., Zhang, X., Burow, M., Payton, P., & Zhang, H. (2013). Expression of the Arabidopsis vacuolar H+-pyrophosphatase gene AVP1 in peanut to improve drought and salt tolerance. Plant Biotechnology Reports, 7(3), 345-355. https://doi.org/10.1007/s11816-012-0269-5