Expression of one of the members of the Arabidopsis chaperonin 60β gene family is developmentally regulated and wound-repressible

Eduardo Zabaleta, Nacyra Assad, Araceli Oropeza, Graciela Salerno, Luis Herrera-Estrella

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

To study the pattern of gene regulation of the plastid chaperonin 60β gene family a chimaeric gene was constructed fusing the 5′-flanking region of the chaperonin 60β B3 gene to the β-glucuronidase reporter gene. Histochemical and fluorometric analysis of the GUS activity present in transgenic plants harbouring this gene construct showed that the B3 promoter is expressed in leaves, stem, petioles and several flower tissues. The pattern of cell type-specific expression in stems and flowers was found to be developmentally regulated. Expression of the B3 promoter was found not to be heat-inducible, but highly repressed by wounding. The rapid decay in GUS activity upon wounding indicates that, at least under some physiological conditions, the gene product of this reporter gene is not as stable as has been previously thought.

Original languageEnglish
Pages (from-to)195-202
Number of pages8
JournalPlant molecular biology
Volume24
Issue number1
DOIs
StatePublished - Jan 1994

Keywords

  • chaperonin 60β
  • gene expression
  • plant transformation
  • wound repression
  • β-glucuronidase

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