Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector

Luis Herrera-Estrella; Ann Depicker; Marc Van Montagu; Jeff Schell

doi:10.1038/303209a0

Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector

Luis Herrera-Estrella, Ann Depicker, Marc Van Montagu, Jeff Schell

Plant & Soil Science

Research output: Contribution to journal › Article › peer-review

388 Scopus citations

Abstract

Foreign genes introduced into plant cells with Ti-plasmid vectors are not expressed. We have constructed an expression vector derived from the promoter sequence of nopaline synthase, and have inserted the coding sequences of the octopine synthase gene and a chloramphenicol acetyltransferase gene into this vector. These chimaeric genes are functionally expressed in plant cells after their transfer via a Ti-plasmid of Agrobacterium tumefaciens.

Original language	English
Pages (from-to)	209-213
Number of pages	5
Journal	Nature
Volume	303
Issue number	5914
DOIs	https://doi.org/10.1038/303209a0
State	Published - 1983

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title = "Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector",

abstract = "Foreign genes introduced into plant cells with Ti-plasmid vectors are not expressed. We have constructed an expression vector derived from the promoter sequence of nopaline synthase, and have inserted the coding sequences of the octopine synthase gene and a chloramphenicol acetyltransferase gene into this vector. These chimaeric genes are functionally expressed in plant cells after their transfer via a Ti-plasmid of Agrobacterium tumefaciens.",

author = "Luis Herrera-Estrella and Ann Depicker and {Van Montagu}, Marc and Jeff Schell",

year = "1983",

doi = "10.1038/303209a0",

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journal = "Nature",

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T1 - Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector

AU - Herrera-Estrella, Luis

AU - Depicker, Ann

AU - Van Montagu, Marc

AU - Schell, Jeff

PY - 1983

Y1 - 1983

N2 - Foreign genes introduced into plant cells with Ti-plasmid vectors are not expressed. We have constructed an expression vector derived from the promoter sequence of nopaline synthase, and have inserted the coding sequences of the octopine synthase gene and a chloramphenicol acetyltransferase gene into this vector. These chimaeric genes are functionally expressed in plant cells after their transfer via a Ti-plasmid of Agrobacterium tumefaciens.

AB - Foreign genes introduced into plant cells with Ti-plasmid vectors are not expressed. We have constructed an expression vector derived from the promoter sequence of nopaline synthase, and have inserted the coding sequences of the octopine synthase gene and a chloramphenicol acetyltransferase gene into this vector. These chimaeric genes are functionally expressed in plant cells after their transfer via a Ti-plasmid of Agrobacterium tumefaciens.

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U2 - 10.1038/303209a0

DO - 10.1038/303209a0

M3 - Article

AN - SCOPUS:0020541709

SN - 0028-0836

VL - 303

SP - 209

EP - 213

JO - Nature

JF - Nature

IS - 5914

ER -

Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector

Abstract

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