Fluorescence spectroscopy and mass spectrometry have been extensively used for characterization of biomaterials, but usually separately. An instrument combining fluorescence spectroscopy and Fourier-transform ion cyclotron resonance mass spectrometry (FTICR-MS) has been developed to explore both fluorescence and mass spectrometric behavior of ions produced by electrospray ionization (ESI) in ultra high vacuum (<5 x 10(-9) mbar). Using rhodamine 6G (R6G) as a sample, the instrument was systematically characterized. Gas-phase fluorescence and mass spectral signal of the same ion population are detected immediately after each other. Effects of gas pressure, ion density, and excitation laser power on the fluorescence signal intensity and mass spectral fragmentation patterns are discussed. Characteristic times of ion photodissociation in ultra high vacuum were recorded for different irradiation powers. Photofragmentation patterns of rhodamine 6G ions in the Penning trap of an FTICR spectro
|Journal||Journal of the American Society for Mass Spectrometry|
|State||Published - Sep 2009|
Chingin, K., Chen, H., Gamez Goytia, G., & Zenobi, R. (2009). Exploring Fluorescence and Fragmentation of Ions Produced by Electrospray Ionization in Ultrahigh Vacuum. Journal of the American Society for Mass Spectrometry, 1731-1738.