Exploring Fluorescence and Fragmentation of Ions Produced by Electrospray Ionization in Ultrahigh Vacuum

Konstantin Chingin, Huanwen Chen, Gerardo Gamez Goytia, Renato Zenobi

Research output: Contribution to journalArticle

Abstract

Fluorescence spectroscopy and mass spectrometry have been extensively used for characterization of biomaterials, but usually separately. An instrument combining fluorescence spectroscopy and Fourier-transform ion cyclotron resonance mass spectrometry (FTICR-MS) has been developed to explore both fluorescence and mass spectrometric behavior of ions produced by electrospray ionization (ESI) in ultra high vacuum (<5 x 10(-9) mbar). Using rhodamine 6G (R6G) as a sample, the instrument was systematically characterized. Gas-phase fluorescence and mass spectral signal of the same ion population are detected immediately after each other. Effects of gas pressure, ion density, and excitation laser power on the fluorescence signal intensity and mass spectral fragmentation patterns are discussed. Characteristic times of ion photodissociation in ultra high vacuum were recorded for different irradiation powers. Photofragmentation patterns of rhodamine 6G ions in the Penning trap of an FTICR spectro
Original languageEnglish
Pages (from-to)1731-1738
JournalJournal of the American Society for Mass Spectrometry
StatePublished - Sep 2009

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