In recent years single molecule detection has evolved from focusing on detecting a single molecule to examining molecular interactions in various systems. This Review discusses the recent progress of single molecule fluorescence detection with respect to biomolecular interactions. Single molecule detection instrumentation is described along with several of the important tools that are used single molecule detection, such as anisotropy and resonance energy transfer. Recent applications of single molecule detection to various types of biomolecular interactions are also reviewed.
|Journal||TrAC: Trends in Analytical Chemistry|
|State||Published - 2007|