Exploring biomolecular interactions by single-molecule fluorescence

Dimitri Pappas, Sean M. Burrows, Randall D. Reif

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


In recent years, single-molecule detection has evolved from focusing on detecting a single molecule to examining molecular interactions in various systems. This review discusses the recent progress of single-molecule-fluorescence detection with respect to biomolecular interactions. We describe single-molecule-detection instrumentation along with several of the important tools that are used (e.g., anisotropy and resonance-energy transfer). We also review recent applications of single-molecule detection to various types of biomolecular interactions.

Original languageEnglish
Pages (from-to)884-894
Number of pages11
JournalTrAC - Trends in Analytical Chemistry
Issue number9
StatePublished - Oct 2007


  • FRET
  • Fluorescence (Förster) resonance-energy transfer
  • Fluorescence anisotropy
  • Fluorescence spectroscopy
  • Lifetime
  • Single cell
  • Single molecule


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