Exploring biomolecular interactions by single-molecule fluorescence

Dimitri Pappas; Sean M. Burrows; Randall D. Reif

doi:10.1016/j.trac.2007.09.003

Exploring biomolecular interactions by single-molecule fluorescence

Dimitri Pappas, Sean M. Burrows, Randall D. Reif

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

In recent years, single-molecule detection has evolved from focusing on detecting a single molecule to examining molecular interactions in various systems. This review discusses the recent progress of single-molecule-fluorescence detection with respect to biomolecular interactions. We describe single-molecule-detection instrumentation along with several of the important tools that are used (e.g., anisotropy and resonance-energy transfer). We also review recent applications of single-molecule detection to various types of biomolecular interactions.

Original language	English
Pages (from-to)	884-894
Number of pages	11
Journal	TrAC - Trends in Analytical Chemistry
Volume	26
Issue number	9
DOIs	https://doi.org/10.1016/j.trac.2007.09.003
State	Published - Oct 2007

Keywords

FRET
Fluorescence (Förster) resonance-energy transfer
Fluorescence anisotropy
Fluorescence spectroscopy
Lifetime
Single cell
Single molecule

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10.1016/j.trac.2007.09.003

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@article{f2b815205ab54e91a21542fb32cb9bc6,

title = "Exploring biomolecular interactions by single-molecule fluorescence",

abstract = "In recent years, single-molecule detection has evolved from focusing on detecting a single molecule to examining molecular interactions in various systems. This review discusses the recent progress of single-molecule-fluorescence detection with respect to biomolecular interactions. We describe single-molecule-detection instrumentation along with several of the important tools that are used (e.g., anisotropy and resonance-energy transfer). We also review recent applications of single-molecule detection to various types of biomolecular interactions.",

keywords = "FRET, Fluorescence (F{\"o}rster) resonance-energy transfer, Fluorescence anisotropy, Fluorescence spectroscopy, Lifetime, Single cell, Single molecule",

author = "Dimitri Pappas and Burrows, {Sean M.} and Reif, {Randall D.}",

note = "Funding Information: The authors thank Horst Vogel and Matthew Paige for providing figures for this article. The authors also acknowledge a grant from the Welch Foundation, which supports many of the single-molecule investigations in their laboratory.",

year = "2007",

month = oct,

doi = "10.1016/j.trac.2007.09.003",

language = "English",

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pages = "884--894",

journal = "TrAC - Trends in Analytical Chemistry",

issn = "0165-9936",

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T1 - Exploring biomolecular interactions by single-molecule fluorescence

AU - Pappas, Dimitri

AU - Burrows, Sean M.

AU - Reif, Randall D.

N1 - Funding Information: The authors thank Horst Vogel and Matthew Paige for providing figures for this article. The authors also acknowledge a grant from the Welch Foundation, which supports many of the single-molecule investigations in their laboratory.

PY - 2007/10

Y1 - 2007/10

N2 - In recent years, single-molecule detection has evolved from focusing on detecting a single molecule to examining molecular interactions in various systems. This review discusses the recent progress of single-molecule-fluorescence detection with respect to biomolecular interactions. We describe single-molecule-detection instrumentation along with several of the important tools that are used (e.g., anisotropy and resonance-energy transfer). We also review recent applications of single-molecule detection to various types of biomolecular interactions.

AB - In recent years, single-molecule detection has evolved from focusing on detecting a single molecule to examining molecular interactions in various systems. This review discusses the recent progress of single-molecule-fluorescence detection with respect to biomolecular interactions. We describe single-molecule-detection instrumentation along with several of the important tools that are used (e.g., anisotropy and resonance-energy transfer). We also review recent applications of single-molecule detection to various types of biomolecular interactions.

KW - FRET

KW - Fluorescence (Förster) resonance-energy transfer

KW - Fluorescence anisotropy

KW - Fluorescence spectroscopy

KW - Lifetime

KW - Single cell

KW - Single molecule

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DO - 10.1016/j.trac.2007.09.003

M3 - Article

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SN - 0165-9936

VL - 26

SP - 884

EP - 894

JO - TrAC - Trends in Analytical Chemistry

JF - TrAC - Trends in Analytical Chemistry

IS - 9

ER -

Exploring biomolecular interactions by single-molecule fluorescence

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Keywords

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