Examining the drought stress transcriptome in cotton leaf and root tissue

Paxton Payton, Kameswara Kottapalli, H. Kebede, James Mahan, Robert Wright, Randy Allen

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Abstract

Growth, yield, and yield quality of cotton are greatly affected by water-deficit stress. We have identified the genes and associated metabolic pathways involved in the water-deficit stress response in leaf and root. Gene expression profiles were developed for leaf and root tissues subjected to slow-onset water deficit under controlled, glasshouse conditions. The water-deficit stress was characterized by leaf water potential of -23.1 bars for stressed tissue compared to -8.7 bars for fully-irrigated control plants and a corresponding decrease in net carbon assimilation to approximately 60% of the rates seen in the irrigated controls (30.3 ± 4.7 μmol CO(2) m(-2) s(-1) compared to 17.8 ± 5.9 μmol CO(2) m(-2) s(-1)). Profiling experiments revealed 2,106 stress-responsive transcripts, 879 classified as stress-induced, 1,163 stress-repressed, and 64 showed reciprocal expression patterns in root and leaf. The majority of stress-responsive transcripts had tissue-specific expression patter
Original languageEnglish
Pages (from-to)821–828
JournalBiotechnol. Lett.
StatePublished - Apr 2011

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Payton, P., Kottapalli, K., Kebede, H., Mahan, J., Wright, R., & Allen, R. (2011). Examining the drought stress transcriptome in cotton leaf and root tissue. Biotechnol. Lett., 821–828.