Evaluations of a mechanistic hypothesis for the influence of extracellular ions on electroporation due to high-intensity, nanosecond pulsing

V. Sridhara; R. P. Joshi

doi:10.1016/j.bbamem.2014.03.010

Evaluations of a mechanistic hypothesis for the influence of extracellular ions on electroporation due to high-intensity, nanosecond pulsing

V. Sridhara, R. P. Joshi

Electrical and Computer Engineering

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The effect of ions present in the extracellular medium on electroporation by high-intensity, short-duration pulsing is studied through molecular dynamic simulations. Our simulation results indicate that mobile ions in the medium might play a role in creating stronger local electric fields across membranes that then reinforce and strengthen electroporation. Much faster pore formation is predicted in higher conductivity media. However, the impact of extracellular conductivity on cellular inflows, which depend on transport processes such as electrophoresis, could be different as discussed here. Our simulation results also show that interactions between cations (Na⁺ in this case) and the carbonyl oxygen of the lipid headgroups could impede pore resealing.

Original language	English
Pages (from-to)	1793-1800
Number of pages	8
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	1838
Issue number	7
DOIs	https://doi.org/10.1016/j.bbamem.2014.03.010
State	Published - Jul 2014

Keywords

Electric pulsing
Ions
Lipid
Molecular dynamics
Nanopore

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10.1016/j.bbamem.2014.03.010

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title = "Evaluations of a mechanistic hypothesis for the influence of extracellular ions on electroporation due to high-intensity, nanosecond pulsing",

abstract = "The effect of ions present in the extracellular medium on electroporation by high-intensity, short-duration pulsing is studied through molecular dynamic simulations. Our simulation results indicate that mobile ions in the medium might play a role in creating stronger local electric fields across membranes that then reinforce and strengthen electroporation. Much faster pore formation is predicted in higher conductivity media. However, the impact of extracellular conductivity on cellular inflows, which depend on transport processes such as electrophoresis, could be different as discussed here. Our simulation results also show that interactions between cations (Na+ in this case) and the carbonyl oxygen of the lipid headgroups could impede pore resealing.",

keywords = "Electric pulsing, Ions, Lipid, Molecular dynamics, Nanopore",

author = "V. Sridhara and Joshi, {R. P.}",

note = "Funding Information: We acknowledge A. Pakhomov (ODU) for useful discussions. Partial support from Old Dominion University is gratefully acknowledged. Finally, we like to acknowledge the Texas Advanced Computing Center and Center for Computational Biology and Bioinformatics for resources in carrying out the MD simulations on multiple processors.",

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AU - Sridhara, V.

AU - Joshi, R. P.

N1 - Funding Information: We acknowledge A. Pakhomov (ODU) for useful discussions. Partial support from Old Dominion University is gratefully acknowledged. Finally, we like to acknowledge the Texas Advanced Computing Center and Center for Computational Biology and Bioinformatics for resources in carrying out the MD simulations on multiple processors.

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AB - The effect of ions present in the extracellular medium on electroporation by high-intensity, short-duration pulsing is studied through molecular dynamic simulations. Our simulation results indicate that mobile ions in the medium might play a role in creating stronger local electric fields across membranes that then reinforce and strengthen electroporation. Much faster pore formation is predicted in higher conductivity media. However, the impact of extracellular conductivity on cellular inflows, which depend on transport processes such as electrophoresis, could be different as discussed here. Our simulation results also show that interactions between cations (Na+ in this case) and the carbonyl oxygen of the lipid headgroups could impede pore resealing.

KW - Electric pulsing

KW - Ions

KW - Lipid

KW - Molecular dynamics

KW - Nanopore

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JF - Biochimica et Biophysica Acta - Biomembranes

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Evaluations of a mechanistic hypothesis for the influence of extracellular ions on electroporation due to high-intensity, nanosecond pulsing

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