Evaluation of an indirect immunofluorescence assay for the detection of Leishmania promastigotes and amastigotes in sand flies and lesion fluid aspirates.

E. W. Mohareb, H. A. Hanafi, E. M. Mikhail, S. M. Presley, R. Batchelor

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Rabbit monospecific antibody Leishmania major surface glycoprotein (gp63) was used in an indirect immunofluorescence assay (IFA) to identify promastigotes and amastigotes from sandflies and lesion fluid aspirates, respectively. Anti-gp63 fluoresced the entire periphery and flagella of promastigotes of different Leishmania species at dilutions to 1:50. Promastigotes were distinctively demonstrated in whole infected sandfly homogenate for 17 days post-infection. Amastigotes from lesion aspirates of infected BALB/c mice and from a human cutaneous leishmaniasis case were also fluoresced by the antibody. This technique could prove to be especially useful in arthropod vector surveillance efforts in which detection of the pathogen is as important as speciation of the sand fly vector.

Original languageEnglish
Pages (from-to)313-321
Number of pages9
JournalJournal of the Egyptian Society of Parasitology
Volume28
Issue number2
StatePublished - Aug 1998

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