Abstract
An indirect cellular enzyme immunoassay for the detection of the serologically defined male-specific antigen, SDMA, was developed using mouse spermatozoa as the target. Serum from B6 female mice injected with male spleen cells was used as the source of SDMA-specific antibody. Our results indicate that the assay is highly reliable (96% accurate) with an intra- and interassay coefficient of variation < 12%. Since this assay is non-subjective and simple to perform it provides a useful alternative to the complement-dependent cytotoxicity assay for detection of SDMA.
Original language | English |
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Pages (from-to) | 121-126 |
Number of pages | 6 |
Journal | Journal of Immunological Methods |
Volume | 142 |
Issue number | 1 |
DOIs | |
State | Published - Aug 28 1991 |
Keywords
- Enzyme immunoassay
- H-Y antigen
- Serologically defined male-specific antigen