Evaluation of a solid-phase cellular enzyme immunoassay for detection of the serologically defined male antigen

Brian D. Reilly; Ellen H. Goldberg

doi:10.1016/0022-1759(91)90299-U

Evaluation of a solid-phase cellular enzyme immunoassay for detection of the serologically defined male antigen

Brian D. Reilly, Ellen H. Goldberg

Biological Sciences

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

An indirect cellular enzyme immunoassay for the detection of the serologically defined male-specific antigen, SDMA, was developed using mouse spermatozoa as the target. Serum from B6 female mice injected with male spleen cells was used as the source of SDMA-specific antibody. Our results indicate that the assay is highly reliable (96% accurate) with an intra- and interassay coefficient of variation < 12%. Since this assay is non-subjective and simple to perform it provides a useful alternative to the complement-dependent cytotoxicity assay for detection of SDMA.

Original language	English
Pages (from-to)	121-126
Number of pages	6
Journal	Journal of Immunological Methods
Volume	142
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(91)90299-U
State	Published - Aug 28 1991

Keywords

Enzyme immunoassay
H-Y antigen
Serologically defined male-specific antigen

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abstract = "An indirect cellular enzyme immunoassay for the detection of the serologically defined male-specific antigen, SDMA, was developed using mouse spermatozoa as the target. Serum from B6 female mice injected with male spleen cells was used as the source of SDMA-specific antibody. Our results indicate that the assay is highly reliable (96% accurate) with an intra- and interassay coefficient of variation < 12%. Since this assay is non-subjective and simple to perform it provides a useful alternative to the complement-dependent cytotoxicity assay for detection of SDMA.",

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AB - An indirect cellular enzyme immunoassay for the detection of the serologically defined male-specific antigen, SDMA, was developed using mouse spermatozoa as the target. Serum from B6 female mice injected with male spleen cells was used as the source of SDMA-specific antibody. Our results indicate that the assay is highly reliable (96% accurate) with an intra- and interassay coefficient of variation < 12%. Since this assay is non-subjective and simple to perform it provides a useful alternative to the complement-dependent cytotoxicity assay for detection of SDMA.

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Evaluation of a solid-phase cellular enzyme immunoassay for detection of the serologically defined male antigen

Abstract

Keywords

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