Evaluation of a solid-phase cellular enzyme immunoassay for detection of the serologically defined male antigen

Brian D. Reilly, Ellen H. Goldberg

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

An indirect cellular enzyme immunoassay for the detection of the serologically defined male-specific antigen, SDMA, was developed using mouse spermatozoa as the target. Serum from B6 female mice injected with male spleen cells was used as the source of SDMA-specific antibody. Our results indicate that the assay is highly reliable (96% accurate) with an intra- and interassay coefficient of variation < 12%. Since this assay is non-subjective and simple to perform it provides a useful alternative to the complement-dependent cytotoxicity assay for detection of SDMA.

Original languageEnglish
Pages (from-to)121-126
Number of pages6
JournalJournal of Immunological Methods
Volume142
Issue number1
DOIs
StatePublished - Aug 28 1991

Keywords

  • Enzyme immunoassay
  • H-Y antigen
  • Serologically defined male-specific antigen

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