TY - JOUR
T1 - Enzyme-linked immunosorbent assay measurement of castor toxin in soils
AU - Boroda, E.
AU - Jaynes, W. F.
AU - Zartman, R. E.
AU - Green, C. J.
AU - San Francisco, M. J.
AU - Zak, J. C.
N1 - Funding Information:
Research supported by the SBCCOM, US Department of Defense under contract DAAD13-00-C-0061. This is an article # T-4-538 from the College of Agricultural Sciences and Natural Resources at Texas Tech University, Lubbock, Texas.
PY - 2004
Y1 - 2004
N2 - Published Enzyme-Linked ImmunoSorbent Assay (ELISA) techniques to determine ricin, a castor (Ricinus communis) toxin, were developed for human sera, biological fluids, and tissue samples. This study was conducted to adapt existing ELISA procedures to soil extracts and demonstrate its use. These modifications of published generic ELISA methods include elimination of phosphate buffers in the soil extraction procedure and addition of bovine Fraction V albumin to block nonspecific sites on the microtiter plates. Using this method with commercially available biochemicals and equipment, it was possible to detect the presence of ricin in a soil after harvest and two years post-harvest.
AB - Published Enzyme-Linked ImmunoSorbent Assay (ELISA) techniques to determine ricin, a castor (Ricinus communis) toxin, were developed for human sera, biological fluids, and tissue samples. This study was conducted to adapt existing ELISA procedures to soil extracts and demonstrate its use. These modifications of published generic ELISA methods include elimination of phosphate buffers in the soil extraction procedure and addition of bovine Fraction V albumin to block nonspecific sites on the microtiter plates. Using this method with commercially available biochemicals and equipment, it was possible to detect the presence of ricin in a soil after harvest and two years post-harvest.
UR - http://www.scopus.com/inward/record.url?scp=3342973892&partnerID=8YFLogxK
U2 - 10.1081/CSS-120030597
DO - 10.1081/CSS-120030597
M3 - Article
AN - SCOPUS:3342973892
SN - 0010-3624
VL - 35
SP - 1185
EP - 1195
JO - Communications in Soil Science and Plant Analysis
JF - Communications in Soil Science and Plant Analysis
IS - 7-8
ER -