Enzyme-linked immunosorbent assay measurement of castor toxin in soils

E. Boroda, W. F. Jaynes, R. E. Zartman, C. J. Green, M. J. San Francisco, J. C. Zak

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Published Enzyme-Linked ImmunoSorbent Assay (ELISA) techniques to determine ricin, a castor (Ricinus communis) toxin, were developed for human sera, biological fluids, and tissue samples. This study was conducted to adapt existing ELISA procedures to soil extracts and demonstrate its use. These modifications of published generic ELISA methods include elimination of phosphate buffers in the soil extraction procedure and addition of bovine Fraction V albumin to block nonspecific sites on the microtiter plates. Using this method with commercially available biochemicals and equipment, it was possible to detect the presence of ricin in a soil after harvest and two years post-harvest.

Original languageEnglish
Pages (from-to)1185-1195
Number of pages11
JournalCommunications in Soil Science and Plant Analysis
Volume35
Issue number7-8
DOIs
StatePublished - 2004

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