Environmental polychlorinated biphenyl exposure and cytochromes P450 in raccoons (Procyon lotor)

Philip N. Smith, Stelvio M. Bandiera, Sherry L. Skipper, Kevin A. Johnson, Scott T. McMurry

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

An investigation involving raccoons as a sentinel species at the Paducah Gaseous Diffusion Plant (PGDP) and Ballard Wildlife Management Area in western Kentucky (USA) delineated the extent of exposure to polychlorinated biphenyls (PCBs). Three separate measures of hepatic cytochrome P450 (CYP) induction were used to evaluate raccoon physiological responses to PCB exposure. Hepatic CYP induction was estimated via determination of total CYP, dealkylase activities, and immunoreactive proteins. There were no differences in raccoon biomarker responses between study sites. Significant relationships between and among PCB residues and biomarkers indicated that hepatic CYP induction had occurred in response to PCB exposure. Pentoxyresorufin O-deethylase (PROD) activity, CYP1A1, and CYP1A2 were biomarkers most closely associated with PCB exposure. The rank order of responses was CYP1A1 > CYP1A2 > PROD > ethoxyresorufin O-deethylase (EROD) as related to raccoon liver PCB concentrations, whereas the order was CYP1A1 > PROD > EROD > CYP1A2 when regressed with total PCB concentrations in abdominal fat.

Original languageEnglish
Pages (from-to)417-423
Number of pages7
JournalEnvironmental Toxicology and Chemistry
Volume22
Issue number2
DOIs
StatePublished - Feb 1 2003

Keywords

  • Biomarker
  • Cytochrome P450
  • Paducah
  • Polychlorinated biphenyls
  • Raccoon

Fingerprint Dive into the research topics of 'Environmental polychlorinated biphenyl exposure and cytochromes P450 in raccoons (Procyon lotor)'. Together they form a unique fingerprint.

Cite this