TY - JOUR
T1 - Enhanced phosphorus uptake in transgenic tobacco plants that overproduce citrate
AU - López-Bucio, José
AU - De la Vega, Octavio Martínez
AU - Guevara-García, Arturo
AU - Herrera-Estrella, Luis
N1 - Funding Information:
We thank Fernanda Nieto, Verenice Ramirez, Omar Ocampo, and Victor Olalde for CS antibodies, mycorrhizal inoculum and technical advice. Drs. Gabriela Olmedo and June Simpson for critical reading of this manuscript. This work was supported in part by grants of the HHMI (75191-526901), the Rockefeller Foundation (AS 9644), and the European Commission (ERBIC-18C-960089) to L.H.E. J.L.B. is a CONACYT fellow No. 113732.
PY - 2000
Y1 - 2000
N2 - Phosphorus (P) is one of the most important nutrients limiting agricultural production worldwide. In acid and alkaline soils, which make up over 70% of the world's arable land, P forms insoluble compounds that are not available for plant use. To reduce P deficiencies and ensure plant productivity, nearly 30 million tons of P fertilizer are applied every year. Up to 80% of the applied P fertilizer is lost because it becomes immobile and unavailable for plant uptake. Therefore, the development of novel plant varieties more efficient in the use of P represents the best alternative to reduce the use of P fertilizers and achieve a more sustainable agriculture. We show here that the ability to use insoluble P compounds can be significantly enhanced by engineering plants to produce more organic acids. Our results show that when compared to the controls, citrate-overproducing plants yield more leaf and fruit biomass when grown under P-limiting conditions and require less P fertilizer to achieve optimal growth.
AB - Phosphorus (P) is one of the most important nutrients limiting agricultural production worldwide. In acid and alkaline soils, which make up over 70% of the world's arable land, P forms insoluble compounds that are not available for plant use. To reduce P deficiencies and ensure plant productivity, nearly 30 million tons of P fertilizer are applied every year. Up to 80% of the applied P fertilizer is lost because it becomes immobile and unavailable for plant uptake. Therefore, the development of novel plant varieties more efficient in the use of P represents the best alternative to reduce the use of P fertilizers and achieve a more sustainable agriculture. We show here that the ability to use insoluble P compounds can be significantly enhanced by engineering plants to produce more organic acids. Our results show that when compared to the controls, citrate-overproducing plants yield more leaf and fruit biomass when grown under P-limiting conditions and require less P fertilizer to achieve optimal growth.
KW - Citrate overproduction
KW - Mycorrhiza
KW - Phosphorus acquisition
KW - Transgenic plants
UR - http://www.scopus.com/inward/record.url?scp=0041073475&partnerID=8YFLogxK
U2 - 10.1038/74531
DO - 10.1038/74531
M3 - Article
C2 - 10748530
AN - SCOPUS:0041073475
SN - 1087-0156
VL - 18
SP - 450
EP - 453
JO - Nature Biotechnology
JF - Nature Biotechnology
IS - 4
ER -