TY - JOUR
T1 - Energy-restricted diet benefits body composition but degrades bone integrity in middle-aged obese female rats
AU - Shen, Chwan Li
AU - Zhu, Wenbin
AU - Gao, Weimin
AU - Wang, Shu
AU - Chen, Lixia
AU - Chyu, Ming Chien
N1 - Funding Information:
This study was supported by Laura W. Bush Institute for Women's Health and Texas Tech University Health Sciences Center. We thank Jay J. Cao for the analyses of femoral bone quality, Raul Y. Dagda for the analyses of mRNA expression and GPX activity, and James K. Yeh for bone histomorphometric analyses.
PY - 2013/8
Y1 - 2013/8
N2 - This study investigates the effects of a restricted diet (RD) on body composition and musculoskeletal health along with endocrines and molecular mechanism in established mature obese rats. Twenty female rats were fed with a high-fat diet (HFD) ad libitum for 4 months and then assigned to either HFD or RD group for another 4 months. Another 10 rats were on a low-fat diet for 8 months. Outcome measures included body composition, bone mineral density, microarchitecrure, and strength; serum leptin, adiponectin, insulin-like growth factor I, and liver glutathione peroxidase activity; and protein expression and spleen tumor necrosis factor α messenger RNA expression. We hypothesized that mature obese rats on a 35% energy restriction diet for 4 months would improve body composition but degrade microstructural and mechanical properties of long bones, and such changes in musculoskeletal integrity are related to the modulation of obesity-related endocrines and proinflammation. Relative to HFD, RD benefited body composition (decreased body weight and %fat mass and increased %fat-free mass); decreased insulin-like growth factor I and leptin; elevated adiponectin, glutathione peroxidase activity and protein expression and tumor necrosis factor α messenger RNA expression; and suppressed bone formation and increased bone resorption, resulting in decreased trabecular and cortical bone volume, bone mineral density, and bone strength. Relative to low-fat diet, RD had a similar effect on body composition and serum markers but increased bone turnover rate and decreased bone mineral density and strength. Our data suggest that long-term RD has a negative impact on bone remodeling in obese female rats, probably through modification of endocrines and elevation of proinflammation.
AB - This study investigates the effects of a restricted diet (RD) on body composition and musculoskeletal health along with endocrines and molecular mechanism in established mature obese rats. Twenty female rats were fed with a high-fat diet (HFD) ad libitum for 4 months and then assigned to either HFD or RD group for another 4 months. Another 10 rats were on a low-fat diet for 8 months. Outcome measures included body composition, bone mineral density, microarchitecrure, and strength; serum leptin, adiponectin, insulin-like growth factor I, and liver glutathione peroxidase activity; and protein expression and spleen tumor necrosis factor α messenger RNA expression. We hypothesized that mature obese rats on a 35% energy restriction diet for 4 months would improve body composition but degrade microstructural and mechanical properties of long bones, and such changes in musculoskeletal integrity are related to the modulation of obesity-related endocrines and proinflammation. Relative to HFD, RD benefited body composition (decreased body weight and %fat mass and increased %fat-free mass); decreased insulin-like growth factor I and leptin; elevated adiponectin, glutathione peroxidase activity and protein expression and tumor necrosis factor α messenger RNA expression; and suppressed bone formation and increased bone resorption, resulting in decreased trabecular and cortical bone volume, bone mineral density, and bone strength. Relative to low-fat diet, RD had a similar effect on body composition and serum markers but increased bone turnover rate and decreased bone mineral density and strength. Our data suggest that long-term RD has a negative impact on bone remodeling in obese female rats, probably through modification of endocrines and elevation of proinflammation.
KW - Body composition
KW - Bone
KW - Energy restriction
KW - Female rat
KW - Musculoskeletal health
KW - Obesity
UR - http://www.scopus.com/inward/record.url?scp=84880706232&partnerID=8YFLogxK
U2 - 10.1016/j.nutres.2013.05.008
DO - 10.1016/j.nutres.2013.05.008
M3 - Article
C2 - 23890357
AN - SCOPUS:84880706232
SN - 0271-5317
VL - 33
SP - 668
EP - 676
JO - Nutrition Research
JF - Nutrition Research
IS - 8
ER -