Elevated levels of hydroxylated phosphocholine lipids in the blood serum of breast cancer patients

Loubna A. Hammad, Guangxiang Wu, Marwa M. Saleh, Iveta Klouckova, Lacey E. Dobrolecki, Robert J. Hickey, Lauren Schnaper, Milos V. Novotny, Yehia Mechref

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

The difference in serum phospholipid content between stage-IV breast cancer patients and diseasefree individuals was studied by employing a combination of chemometric statistical analysis tools and mass spectrometry. Chloroform-extracted serum samples were profiled for their lipid class composition and structure using precursor ion, neutral loss, and product ion tandem mass spectrometric (MS/MS) scanning experiments. Changes in the relative abundance of phospholipids in serum as a consequence of cancer progression, measured through electrospray ionization (ESI) mass spectrometry of flow-injected serum samples collected from 25 disease-free individuals and 50 patients diagnosed with stage-IV breast cancer, were statistically evaluated using principal component analysis (PCA), analysis of variance (ANOVA) and receiver operating characteristic (ROC) analysis. Lipids whose abundance changed significantly as a consequence of cancer progression were structurally characterized using product ion spectra, and independently quantified using precursor ion scan experiments against an internal standard of known concentration. Phosphocholine lipids that displayed a statistically significant change as a consequence of cancer progression were found to contain an oxidized fatty acid moiety as determined by MS 3 experiments.

Original languageEnglish
Pages (from-to)863-876
Number of pages14
JournalRapid Communications in Mass Spectrometry
Volume23
Issue number6
DOIs
StatePublished - Jun 23 2009

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