Electrospray ionization mass spectroscopic analysis of peptides modified with N-ethylmaleimide or iodoacetanilide

Masoud Zabet-Moghaddam, Tomoko Kawamura, Emi Yatagai, Satomi Niwayama

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The cysteine-specific modifiers we reported previously, N-ethylmaleimide (NEM) and iodoacetanilide (IAA), have been applied to label cysteine residues of peptides in combination with electrospray ionization mass spectrometry (ESI-MS/MS), and their scope in proteomic studies was examined. Peptides modified with N-ethylmaleimide (NEM) or iodoacetanilide (IAA) showed significant enhancement in ionization efficiencies. These modifiers were also found to remain intact in tandem mass spectrometry. Both combinations of N-ethylmaleimide (NEM) and d5-N-ethylmaleimide (d5-NEM), and iodoacetanilide (IAA) and 13C6-iodoacetanilide (13C6-IAA) were also shown to be applicable to quantitative analysis of a peptide.

Original languageEnglish
Pages (from-to)4891-4895
Number of pages5
JournalBioorganic and Medicinal Chemistry Letters
Volume18
Issue number17
DOIs
StatePublished - Sep 1 2008

Keywords

  • Amino acid modifiers
  • Mass spectrometry
  • Peptides
  • Proteomics

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