TY - JOUR
T1 - Electrospray ionization mass spectroscopic analysis of peptides modified with N-ethylmaleimide or iodoacetanilide
AU - Zabet-Moghaddam, Masoud
AU - Kawamura, Tomoko
AU - Yatagai, Emi
AU - Niwayama, Satomi
N1 - Funding Information:
This work is supported by the Texas Tech University-Texas Tech University Health Sciences Center Joint Initiative Grants.
PY - 2008/9/1
Y1 - 2008/9/1
N2 - The cysteine-specific modifiers we reported previously, N-ethylmaleimide (NEM) and iodoacetanilide (IAA), have been applied to label cysteine residues of peptides in combination with electrospray ionization mass spectrometry (ESI-MS/MS), and their scope in proteomic studies was examined. Peptides modified with N-ethylmaleimide (NEM) or iodoacetanilide (IAA) showed significant enhancement in ionization efficiencies. These modifiers were also found to remain intact in tandem mass spectrometry. Both combinations of N-ethylmaleimide (NEM) and d5-N-ethylmaleimide (d5-NEM), and iodoacetanilide (IAA) and 13C6-iodoacetanilide (13C6-IAA) were also shown to be applicable to quantitative analysis of a peptide.
AB - The cysteine-specific modifiers we reported previously, N-ethylmaleimide (NEM) and iodoacetanilide (IAA), have been applied to label cysteine residues of peptides in combination with electrospray ionization mass spectrometry (ESI-MS/MS), and their scope in proteomic studies was examined. Peptides modified with N-ethylmaleimide (NEM) or iodoacetanilide (IAA) showed significant enhancement in ionization efficiencies. These modifiers were also found to remain intact in tandem mass spectrometry. Both combinations of N-ethylmaleimide (NEM) and d5-N-ethylmaleimide (d5-NEM), and iodoacetanilide (IAA) and 13C6-iodoacetanilide (13C6-IAA) were also shown to be applicable to quantitative analysis of a peptide.
KW - Amino acid modifiers
KW - Mass spectrometry
KW - Peptides
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=49949098404&partnerID=8YFLogxK
U2 - 10.1016/j.bmcl.2008.07.069
DO - 10.1016/j.bmcl.2008.07.069
M3 - Article
C2 - 18701283
AN - SCOPUS:49949098404
SN - 0960-894X
VL - 18
SP - 4891
EP - 4895
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 17
ER -