Effects of recombinant bovine somatotropin administration at breeding on cow, conceptus, and subsequent offspring performance of beef cattle

V. R.G. Mercadante, P. L.P. Fontes, F. M. Ciriaco, D. D. Henry, P. Moriel, A. D. Ealy, S. E. Johnson, N. Di Lorenzo, G. C. Lamb

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Abstract

The effects of administration of recombinant bovine ST (bST) on plasma hormone concentrations of cows, conceptus development, and postnatal calf performance were examined. Lactating beef cows (n = 190) were exposed to a fixed-time AI (TAI) protocol from d −10 to 0 (TAI on d 0). Cows were blocked by breed and stratified by days postpartum and then randomly assigned to receive, subcutaneously 1) 2 injections of saline (1 mL of 0.9% saline), 1 on d 0 at TAI and a second injection on d 14 (CTRL; n = 53); 2) an injection of 325 mg of bST on d 0 and a saline injection on d 14 (bST0; n = 48); 3) a saline injection on d 0 and an injection of 325 mg of bST on d 14 (bST14; n = 49); or 4) 2 injections of 325 mg of bST, 1 on d 0 and a second injection on d 14 (bST0+14; n = 40). Pregnancy status, crown-to-rump length (CRL) on Day 35, and crown-to-nose length (CNL) on Day 65 were determined via transrectal ultrasonography. Blood samples were collected on d 0, 7, 14, 21, 35, and 65, relative to TAI, to determine plasma concentrations of progesterone (P4), IGF-1, and pregnancy-specific protein B (PSPB) and also on d 18 and 21 for isolation of peripheral blood leukocytes for RNA extraction and measurement of interferon-stimulated genes transcript abundance. Individual calf BW was determined at birth and every 30 d until weaning. A subset of 24 calves was randomly selected for liver biopsies at birth to determine mRNA expression of target genes. Administration of bST to cows increased (P < 0.0001) concentrations of plasma IGF-1 for 14 d after injection compared with CTRL but did not affect fetal CRL and CNL (P = 0.23). Cows receiving bST only on d 0 had a greater (P = 0.05) transcript abundance in myxovirus resistance 2 on d 21 compared with 2bST cows (2.0- and 0.8-fold for bST0 and 2bST, respectively), whereas cows receiving bST14 and CTRL were intermediate (1.2- and 0.9-fold, respectively). Calf BW did not differ (P ≥ 0.100) among treatments on d 0, 30, 60, 90, 120, and 150 relative to birth. Injection of bST only on d 0 tended (P = 0.062) to increase calf liver mRNA expression of IGF-1 receptor at birth compared with the calves born to cows in other treatments. Therefore, during a TAI protocol, the administration of 1 or 2 injections of 325 mg of bST to lactating beef cows enhanced their plasma concentrations of IGF-1 but failed to improve fetal size and plasma concentrations of maternal PSPB and P4 and had no effect on postnatal calf growth performance.

Original languageEnglish
Pages (from-to)2128-2138
Number of pages11
JournalJournal of animal science
Volume94
Issue number5
DOIs
StatePublished - May 2016

Keywords

  • Beef cattle
  • Fetal programming
  • Insulin-like growth factor 1
  • Recombinant bovine somatotropin

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    Mercadante, V. R. G., Fontes, P. L. P., Ciriaco, F. M., Henry, D. D., Moriel, P., Ealy, A. D., Johnson, S. E., Di Lorenzo, N., & Lamb, G. C. (2016). Effects of recombinant bovine somatotropin administration at breeding on cow, conceptus, and subsequent offspring performance of beef cattle. Journal of animal science, 94(5), 2128-2138. https://doi.org/10.2527/jas.2015-0217