Effects of patulin on postimplantation rat embryos

Ernest Smith; Eustae A. Duffus; Michele H. Small

Effects of patulin on postimplantation rat embryos

Ernest Smith, Eustae A. Duffus, Michele H. Small

Environmental Toxicology

Research output: Contribution to journal › Article › peer-review

Abstract

Patulin (PAT), a highly toxic, carcinogenic, heterocyclic lactone is produced by a variety of fungal species, including Penicillium and Aspergillus. This compound has been isolated from various apple products and is stable in apple and grape juice and dry corn. It has been reported to be cytotoxic and to exert adverse influence on development in vivo in mice and merits further study and evaluation. In this study, whole rate embryo culture (WEC) was used to determine the teratogenic potential of PAT in vitro. Embryos were exposed to PAT-treated (0.00-62 microM) rat serum for 45 h. The embryos that were exposed to 62 microM PAT were not evaluated because they did not survive beyond 40 h of incubation. The results indicate that PAT induced a statistically significant reduction in protein and DNA content, yolk sac diameter, crown rump length, and somite number count. Patulin treatment also resulted in an increase in the frequency of defective embryos. Anomalies included growth retardation

Original language	English
Pages (from-to)	267-70
Journal	Arch Environ Contam Toxicol
State	Published - Aug 1 1993

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title = "Effects of patulin on postimplantation rat embryos",

abstract = "Patulin (PAT), a highly toxic, carcinogenic, heterocyclic lactone is produced by a variety of fungal species, including Penicillium and Aspergillus. This compound has been isolated from various apple products and is stable in apple and grape juice and dry corn. It has been reported to be cytotoxic and to exert adverse influence on development in vivo in mice and merits further study and evaluation. In this study, whole rate embryo culture (WEC) was used to determine the teratogenic potential of PAT in vitro. Embryos were exposed to PAT-treated (0.00-62 microM) rat serum for 45 h. The embryos that were exposed to 62 microM PAT were not evaluated because they did not survive beyond 40 h of incubation. The results indicate that PAT induced a statistically significant reduction in protein and DNA content, yolk sac diameter, crown rump length, and somite number count. Patulin treatment also resulted in an increase in the frequency of defective embryos. Anomalies included growth retardation",

author = "Ernest Smith and Duffus, {Eustae A.} and Small, {Michele H.}",

year = "1993",

month = aug,

day = "1",

language = "English",

pages = "267--70",

journal = "Arch Environ Contam Toxicol",

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TY - JOUR

T1 - Effects of patulin on postimplantation rat embryos

AU - Smith, Ernest

AU - Duffus, Eustae A.

AU - Small, Michele H.

PY - 1993/8/1

Y1 - 1993/8/1

N2 - Patulin (PAT), a highly toxic, carcinogenic, heterocyclic lactone is produced by a variety of fungal species, including Penicillium and Aspergillus. This compound has been isolated from various apple products and is stable in apple and grape juice and dry corn. It has been reported to be cytotoxic and to exert adverse influence on development in vivo in mice and merits further study and evaluation. In this study, whole rate embryo culture (WEC) was used to determine the teratogenic potential of PAT in vitro. Embryos were exposed to PAT-treated (0.00-62 microM) rat serum for 45 h. The embryos that were exposed to 62 microM PAT were not evaluated because they did not survive beyond 40 h of incubation. The results indicate that PAT induced a statistically significant reduction in protein and DNA content, yolk sac diameter, crown rump length, and somite number count. Patulin treatment also resulted in an increase in the frequency of defective embryos. Anomalies included growth retardation

AB - Patulin (PAT), a highly toxic, carcinogenic, heterocyclic lactone is produced by a variety of fungal species, including Penicillium and Aspergillus. This compound has been isolated from various apple products and is stable in apple and grape juice and dry corn. It has been reported to be cytotoxic and to exert adverse influence on development in vivo in mice and merits further study and evaluation. In this study, whole rate embryo culture (WEC) was used to determine the teratogenic potential of PAT in vitro. Embryos were exposed to PAT-treated (0.00-62 microM) rat serum for 45 h. The embryos that were exposed to 62 microM PAT were not evaluated because they did not survive beyond 40 h of incubation. The results indicate that PAT induced a statistically significant reduction in protein and DNA content, yolk sac diameter, crown rump length, and somite number count. Patulin treatment also resulted in an increase in the frequency of defective embryos. Anomalies included growth retardation

M3 - Article

SP - 267

EP - 270

JO - Arch Environ Contam Toxicol

JF - Arch Environ Contam Toxicol

ER -