Effects of in vitro adrenocorticotrophic hormone, cortisol and human recombinant interleukin-2 on porcine neutrophil migration and luminol-dependent chemiluminescence

J. L. Salak, J. J. McGlone, M. Lyte

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41 Scopus citations

Abstract

Experiments were performed on isolated neutrophils tested in vitro to investigate the role of adrenocorticotrophic hormone (ACTH), cortisol and recombinant human interleukin-2 (rhIL-2) in modulating porcine polymorphonuclear (PMN) leukocyte function. Elevated levels of cortisol, similar to that observed during stressful experiences, caused a decrease in PMN chemokinesis (P < 0.02), chemotaxis (P < 0.001) and luminol-dependent chemiluminescence. ACTH at concentrations reflective of high stress conditions reduced PMN chemotaxis (P < 0.02) and chemokinesis (P < 0.01). In vitro rhIL-2 reversed (P < 0.05) the cortisol-induced and ACTH-induced suppression of PMN chemokinesis. These data indicate that specific neutrophil functions in pigs may be impaired by stress-related hormones of the pituitary-adrenal axis and that IL-2 may reverse some endocrine-induced suppression in chemokinesis.

Original languageEnglish
Pages (from-to)327-337
Number of pages11
JournalVeterinary Immunology and Immunopathology
Volume39
Issue number4
DOIs
StatePublished - Dec 1993

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