Effects of glucosamine and chondroitin sulfate on mediators of osteoarthritis in cultured equine chondrocytes stimulated by use of recombinant equine interleukin-1β

Kirsten M. Neil, Michael W. Orth, Paul M. Coussens, Pooi See Chan, John P. Caron

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32 Scopus citations

Abstract

Objective - To determine whether glucosamine and chondroitin sulfate (CS) at concentrations approximating those achieved in plasma by oral administration would influence gene expression of selected mediators of osteoarthritis in cytokine-stimulated equine articular chondrocytes. Sample population - Samples of grossly normal articular cartilage obtained from the metacarpophalangeal joint of 13 horses. Procedure - Equine chondrocytes in pellet culture were stimulated with a subsaturating dose of recombinant equine interleukin (reIL)-1β. Effects of prior incubation with glucosamine (2.5 to 10.0 μg/mL) and CS (5.0 to 50.0 μg/mL) on gene expression of matrix metalloproteinase (MMP)-1, -2, -3, -9, and -13; aggrecanase 1 and 2; inducible nitric oxide synthase (iNOS); cyclooxygenase (COX)-2; nuclear factor κB; and c-Jun-N-terminal kinase (JNK) were assessed by use of a quantitative real-time polymerase chain reaction assay. Results - Glucosamine at a concentration of 10 μg/mL significantly reduced reIL-1β-induced mRNA expression of MMP-13, aggrecanase 1, and JNK. Reductions in cytokine-induced expression were also observed for iNOS and COX-2. Chondroitin sulfate had no effect on gene expression at the concentrations tested. Conclusions and clinical relevance - Concentrations of glucosamine similar to those achieved in plasma after oral administration in horses exerted pretranslational regulation of some mediators of osteoarthritis, an effect that may contribute to the cartilage-sparing properties of this aminomonosaccharide. Analysis of results of this study indicated that the influence of CS on pretranslational regulation of these selected genes is limited or lacking.

Original languageEnglish
Pages (from-to)1861-1869
Number of pages9
JournalAmerican Journal of Veterinary Research
Volume66
Issue number11
DOIs
StatePublished - Nov 2005

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