TY - JOUR
T1 - Effects of environmentally relevant concentrations of 2,3,7,8-TCDD on domestic chicken immune function and CYP450 activity
T2 - F1 generation and egg injection studies
AU - Peden-Adams, M.
AU - Alonso, K.
AU - Godard, C.
AU - Skipper, S.
AU - Mashburn, W.
AU - Hoover, J.
AU - Charbonneau, C.
AU - Henshel, D.
AU - Dickerson, R.
N1 - Funding Information:
ACKNOWLEDGMENTS This research was funded by the U.S. Fish and Wildlife Service, Columbia, MO, The authors would like to thank Drs. S. Sheffield and A.B. Bodine for early reviews of this manuscript and J. Y. Liu for assistance with revisions. We are grateful to S. Kennedy, M. Ronis, M. Melancon, and R. Norstrom for their insight. We are also appreciative of all the help Kathy Bryant, Rachel Seifer, and Ron Gosset provided for this project. Clemson University extension publication #435 1.
PY - 1998/10
Y1 - 1998/10
N2 - Domestic chickens (Gallus gallus) were used as a surrogate species for wild turkey to assess risk from environmental 2,3,7,8-TCDD exposure. Lymphocyte proliferation and CYP450 induction were assessed in adults exposed via i.m. injection, in F1 14-day old hatchlings, in F1 adults (30-weeks old), and in 14-day old hatchlings exposed via yolk sac injections. Hatchlings from injected eggs exhibited a dose-response in lymphocyte proliferation, IgM titers, EROD, and PROD endpoints. Exposed adults showed a significant dose-dependent increase in CYP450 induction. F1 14-day old chicks exhibited a significant dose-dependent suppression of B-cell proliferation and induction of CYP450 enzymes. F1 adult proliferative responses exhibited B-cell suppression, that was not statistically significant. Significant sex-dependent EROD and MROD induction was also observed in F1 adults, indicating mixed-function oxidase imprinting from maternal exposure.
AB - Domestic chickens (Gallus gallus) were used as a surrogate species for wild turkey to assess risk from environmental 2,3,7,8-TCDD exposure. Lymphocyte proliferation and CYP450 induction were assessed in adults exposed via i.m. injection, in F1 14-day old hatchlings, in F1 adults (30-weeks old), and in 14-day old hatchlings exposed via yolk sac injections. Hatchlings from injected eggs exhibited a dose-response in lymphocyte proliferation, IgM titers, EROD, and PROD endpoints. Exposed adults showed a significant dose-dependent increase in CYP450 induction. F1 14-day old chicks exhibited a significant dose-dependent suppression of B-cell proliferation and induction of CYP450 enzymes. F1 adult proliferative responses exhibited B-cell suppression, that was not statistically significant. Significant sex-dependent EROD and MROD induction was also observed in F1 adults, indicating mixed-function oxidase imprinting from maternal exposure.
UR - http://www.scopus.com/inward/record.url?scp=0032192276&partnerID=8YFLogxK
U2 - 10.1016/S0045-6535(98)00259-8
DO - 10.1016/S0045-6535(98)00259-8
M3 - Article
AN - SCOPUS:0032192276
SN - 0045-6535
VL - 37
SP - 1923
EP - 1939
JO - Chemosphere
JF - Chemosphere
IS - 9-12
ER -