Effect of two major N-nitroso hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) metabolites on earthworm reproductive success

Baohong Zhang, Stephen B. Cox, Scott T. McMurry, W. Andrew Jackson, George P. Cobb, Todd A. Anderson

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Soil and topical tests were employed to investigate the effect of two N-nitroso metabolites of RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine) on earthworm reproduction. The lowest observed effect concentration (LOEC) for cocoon production and hatching was 50 mg/kg for both hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine (MNX) and hexahydro-1,3,5-trinitroso-1,3,5-triazine (TNX) in soil. MNX and TNX also significantly affected cocoon hatching in soil (p < 0.001) and in topical tests (p = 0.001). The LOECs for cocoon hatching were 1 and 10 mg/kg for MNX and TNX in soil, respectively, and 10 mg/L in the topical test. Greater than 100 mg/kg MNX and TNX completely inhibited cocoon hatching. In soil, the EC20 values for MNX were 8.7 and 8.8 mg/kg for cocoon and juvenile production, respectively, compared to 9.2 and 9.1 mg/kg for TNX, respectively. The EC20 values for the total number of cocoon hatchlings were 3.1 and 4.7 mg/kg for MNX and TNX, respectively, in soil and 4.5 and 3.1 mg/L in the topical test. Both MNX and TNX inhibited cocoon production and hatching, suggesting that they may have a negative affect on soil ecosystems at contaminated sites.

Original languageEnglish
Pages (from-to)658-667
Number of pages10
JournalEnvironmental Pollution
Volume153
Issue number3
DOIs
StatePublished - Jun 2008

Keywords

  • Earthworm
  • Hexahydro-1,3,5-trinitro-1,3,5-triazine
  • MNX
  • N-nitroso compounds
  • RDX
  • Reproduction
  • TNX
  • Toxicity

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