Effect of somatostatins and insulin on blood glucose levels of larvae and metamorphosing landlocked sea lamprey, petromyzon marinus

Yung Hsi Kao, John H. Youson, John A. Holmes, Mark A. Sheridan

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

We examined the effects of insulin (INS) and somatostatin 14 (SS-14) on glycemic regulation in larval and metamorphosing landlocked sea lamprey, Petromyzon marinus. Animals were injected intraperitoneally with either (Experiment 1) saline (0.6%), somatostatin-14 (SS-14; 50 or 500 ng/g body weight), insulin (INS; 10 or 100 ng/g body weight), or alloxan (20 or 200 μg/g body weight), or with (Experiment 2) normal rabbit serum, anti-SS-14, anti-lamprey SS-34 or anti-lamprey INS. Somatostatin-14 (500 ng/g) increased plasma glucose levels in larvae. Injection of anti-SS-14 and anti-lamprey SS-34 resulted in hypoglycemia compared to the controls. Insulin (100 ng/g) resulted in hypoglycemia in both larvae and stage 6 metamorphosing lamrpey. Acute insufficiency of lamprey INS in larvae treated with anti-lamprey INS elevated plasma glucose levels. Similarly, alloxan (200 μg/g, a cytotoxin of insulin-secreting cells) resulted in hyperglycemia in larvae. These data indicate that SS-14 is hyperglycemic in sea lamprey, whereas INS is hypoglycemic, and suggest that the glucoregulatory roles of SS-14 and INS emerged early during the evolution of vertebrates.

Original languageEnglish
Pages (from-to)1113-1116
Number of pages4
JournalZoological Science
Volume18
Issue number8
DOIs
StatePublished - Nov 2001

Keywords

  • Glucose homeostasis
  • Insulin
  • Lamprey
  • Metamorphosis
  • Somatostatin

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