Effect of Lys(-20) substitutions in alkaline phosphatase signal peptide on secretion of the enzyme

Z. N. Karamysheva, A. L. Karamyshev, V. N. Ksenzenko, M. A. Nesmeyanova

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The effect of substitutions for positively charged Lys(-20) in the N-terminal domain of the E. coli alkaline phosphatase signal peptide on the enzyme secretion has been studied. The amber-suppressor method was used to obtain mutant alkaline phosphatases: amber mutation was introduced in the appropriate position of the alkaline phosphatase gene using oligonucleotide-directed mutagenesis, and mutant proteins were synthesized in E. coli strains producing amber-suppressor tRNAs specific for Tyr, Gly, Ala, Glu, Phe, His, Cys, and Pro. All the mutant proteins can be translocated across the cytoplasmic membrane and assembled in the periplasm to active enzyme molecules. However, certain amino acid substitutions reduce the rate of protein maturation. Their effect depends not only on the charge of the amino acid residue, but also on its nature. Thus, introduction of positively charged His and the polar uncharged Tyr has no significant effect, while introduction of negatively charged Glu and the hydrophobic Ala, Phe, and Pro, as well as Gly and Cys, has an inhibiting action. The data show the important role of the primary structure of the signal peptide N-terminus in secretion.

Original languageEnglish
Pages (from-to)541-548
Number of pages8
JournalBiochemistry (Moscow)
Volume61
Issue number4
StatePublished - 1996

Keywords

  • Alkaline phosphatase
  • Amino acid substitutions
  • E. coli
  • Protein secretion
  • Signal peptide
  • Suppressor tRNA

Fingerprint Dive into the research topics of 'Effect of Lys(-20) substitutions in alkaline phosphatase signal peptide on secretion of the enzyme'. Together they form a unique fingerprint.

  • Cite this