Effect of L-dopa on interleukin-1β-induced suppression of luteinizing hormone secretion in intact female rats

M. P. Sirivelu, A. C. Shin, G. I. Perez, P. S. MohanKumar, S. M.J. MohanKumar

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

BACKGROUND: The cytokine, interleukin-1β (IL-1β), increases during immune stress and is known to suppress the preovulatory luteinizing hormone (LH) surge in female rats by decreasing hypothalamic norepinephrine (NE). We hypothesized that IL-1β could produce this effect by decreasing NE biosynthesis. METHODS: Female Sprague-Dawley rats were implanted with a push-pull cannula in the medial preoptic area (MPA) of the hypothalamus and a catheter in the jugular vein. They were treated i.p. with the vehicle or 5 μg of IL-1β, the NE precursor, L-dopa, or a combination of L-dopa and IL-1β at 1300 hours on the day of proestrus. They were subjected to push-pull perfusion and serial blood sampling. Perfusates were analyzed for NE levels and serum samples for LH. RESULTS: IL-1β treatment blocked the increase in NE levels in the MPA and the LH surge. Treatment with L-dopa was able to partially restore both NE and LH levels during the afternoon of proestrus. IL-1β treatment caused failure of ovulation and this effect was also reversed by L-dopa. CONCLUSIONS: These results suggest that IL-1β could decrease NE levels in the MPA to suppress reproductive functions and L-dopa can be used to counter this effect.

Original languageEnglish
Pages (from-to)718-725
Number of pages8
JournalHuman Reproduction
Volume24
Issue number3
DOIs
StatePublished - Mar 2009

Keywords

  • Hypothalamus
  • IL-1β
  • L-dopa
  • Luteinizing hormone

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