Dynamical Modeling of Cellular Response to Short-Duration, High-Intensity Electric Fields

Ravindra P. Joshi, Qin Hu, Karl H. Schoenbach

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

The interaction of ultra-short duration, high-intensity electric fields with biological cells has recently begun to generate significant interest due to the possibility for non-thermal manipulation of cellular functions. It is clear that a full understanding requires a dynamical model for both electroporation and the electrostatic potential evolution. Here, dynamical aspects related to electroporation are reviewed. The simple model used in the literature is somewhat incorrect and unphysical for a variety of reasons. Our model for the pore formation energy, E(r), includes a dependence on pore population, density, a variable surface tension, and is dynamic in nature. It is shown that membranes can survive a strong electric pulse and recover provided the pore distribution has a relatively large spread. If, however, the population consists predominantly of larger radii pores, then irreversibility can result. Physically, such a distribution could arise if pores at adjacent sites coalesce. Results show that a finite time delay exists for pore formation, and can lead to a transient overshoot of the transmembrane potential V mem beyond 1.0 V. Pore resealing is shown to consist of an initial fast process, a 10-4 s delay, followed by a much slower closing at a time constant of about 10-1 s. This establishes a time-window for effective killing by a second pulse.

Original languageEnglish
Pages (from-to)778-787
Number of pages10
JournalIEEE Transactions on Dielectrics and Electrical Insulation
Volume10
Issue number5
DOIs
StatePublished - Oct 2003

Keywords

  • Cell response
  • Dynamic
  • Electroporation
  • Pulsed field
  • Smoluchowski model

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