Does F1-ATPase have a catalytic site that preferentially binds MgADP?

Hui Z. Mao; Wesley D. Gray; Joachim Weber

doi:10.1016/j.febslet.2006.06.059

Does F₁-ATPase have a catalytic site that preferentially binds MgADP?

Hui Z. Mao, Wesley D. Gray, Joachim Weber

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

During ATP synthesis, ATP synthase has to bind MgADP in the presence of an excess of MgATP. Thus, for efficient ATP synthesis it would be desirable if incoming substrate could be bound to a catalytic site with a preference for MgADP over MgATP. We tested three hypotheses predicting the existence of such a site. However, our results showed that, at least in absence of an electrochemical proton gradient, none of the three catalytic sites has a higher affinity for MgADP than for MgATP.

Original language	English
Pages (from-to)	4131-4135
Number of pages	5
Journal	FEBS Letters
Volume	580
Issue number	17
DOIs	https://doi.org/10.1016/j.febslet.2006.06.059
State	Published - Jul 24 2006

Keywords

ATP synthase
Binding change
Catalytic sites
Enzyme mechanism
F-ATPase
High-affinity site

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10.1016/j.febslet.2006.06.059

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@article{0c85a949c2124d05b872f22375339173,

title = "Does F1-ATPase have a catalytic site that preferentially binds MgADP?",

abstract = "During ATP synthesis, ATP synthase has to bind MgADP in the presence of an excess of MgATP. Thus, for efficient ATP synthesis it would be desirable if incoming substrate could be bound to a catalytic site with a preference for MgADP over MgATP. We tested three hypotheses predicting the existence of such a site. However, our results showed that, at least in absence of an electrochemical proton gradient, none of the three catalytic sites has a higher affinity for MgADP than for MgATP.",

keywords = "ATP synthase, Binding change, Catalytic sites, Enzyme mechanism, F-ATPase, High-affinity site",

author = "Mao, {Hui Z.} and Gray, {Wesley D.} and Joachim Weber",

note = "Funding Information: Supported by NIH Grant GM071462. J.W. would like to thank Drs. Ina L. Urbatsch and Harry M. Weitlauf for their support during his stay at TTUHSC. The authors thank Dr. Alan E. Senior for helpful discussions.",

year = "2006",

month = jul,

day = "24",

doi = "10.1016/j.febslet.2006.06.059",

language = "English",

volume = "580",

pages = "4131--4135",

journal = "FEBS Letters",